{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:26:16Z","timestamp":1761647176906,"version":"3.30.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A total of 153 strains of<jats:italic>Listeria monocytogenes<\/jats:italic>isolated from different sources (72 from sheep, 12 from cattle, 18 from feedstuffs, and 51 from humans) in Spain from 1989 to 2000 were characterized by pulsed-field gel electrophoresis. The strains of<jats:italic>L. monocytogenes<\/jats:italic>displayed 55 pulsotypes. The 84 animal, 51 human, and 18 feedstuff strains displayed 31, 29, and 7 different pulsotypes, respectively, indicating a great genetic diversity among the Spanish<jats:italic>L. monocytogenes<\/jats:italic>isolates studied.<jats:italic>L. monocytogenes<\/jats:italic>isolates from clinical samples and feedstuffs consumed by the diseased animals were analyzed in 21 flocks. In most cases, clinical strains from different animals of the same flock had identical pulsotypes, confirming the existence of a listeriosis outbreak.<jats:italic>L. monocytogenes<\/jats:italic>strains with pulsotypes identical to those of clinical strains were isolated from silage, potatoes, and maize stalks. This is the first study wherein potatoes and maize stalks are epidemiologically linked with clinical listeriosis.<\/jats:p>","DOI":"10.1128\/aem.67.12.5840-5843.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"5840-5843","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Molecular Typing by Pulsed-Field Gel Electrophoresis of Spanish Animal and Human<i>Listeria monocytogenes<\/i>Isolates"],"prefix":"10.1128","volume":"67","author":[{"given":"A. I.","family":"Vela","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Sanidad Animal (Patolog\u0131\u0301a Animal I), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Fernandez-Garayzabal","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Sanidad Animal (Patolog\u0131\u0301a Animal I), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Vazquez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Servicio de Bacteriolog\u0131\u0301a, Centro Nacional de Microbiolog\u0131\u0301a-Instituto de Salud Carlos III, Majadahonda, 28220 Madrid,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Latre","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza,3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M.","family":"Blanco","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Sanidad Animal (Patolog\u0131\u0301a Animal I), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Moreno","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Sanidad Animal (Patolog\u0131\u0301a Animal I), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"de la Fuente","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Servicio de Bacteriolog\u0131\u0301a, Centro Nacional de Microbiolog\u0131\u0301a-Instituto de Salud Carlos III, Majadahonda, 28220 Madrid,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Marco","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Laboratorio Normativo de Salud Pu\u0301blica. C. M. 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