{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:00:07Z","timestamp":1791338407054,"version":"4.3.1"},"reference-count":79,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A total of 26 strains of\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            , including members of the O1, O139, and non-O1, non-O139 serogroups from both clinical and environmental sources, were examined for the presence of genes encoding cholera toxin (\n            <jats:italic>ctx<\/jats:italic>\n            A), zonula occludens toxin (\n            <jats:italic>zot<\/jats:italic>\n            ), accessory cholera enterotoxin (\n            <jats:italic>ace<\/jats:italic>\n            ), hemolysin (\n            <jats:italic>hly<\/jats:italic>\n            A), NAG-specific heat-stable toxin (\n            <jats:italic>st<\/jats:italic>\n            ), toxin-coregulated pilus (\n            <jats:italic>tcp<\/jats:italic>\n            A), and outer membrane protein (\n            <jats:italic>omp<\/jats:italic>\n            U), for genomic organization, and for the presence of the regulatory protein genes\n            <jats:italic>tcp<\/jats:italic>\n            I and\n            <jats:italic>tox<\/jats:italic>\n            R in order to determine relationships between epidemic serotypes and sources of isolation. While 22 of the 26 strains were hemolytic on 5% sheep blood nutrient agar, all strains were PCR positive for\n            <jats:italic>hly<\/jats:italic>\n            A, the hemolysin gene. When multiplex PCR was used, all serogroup O1 and O139 strains were positive for\n            <jats:italic>tcp<\/jats:italic>\n            A,\n            <jats:italic>omp<\/jats:italic>\n            U, and\n            <jats:italic>tcp<\/jats:italic>\n            I. All O1 and O139 strains except one O1 strain and one O139 strain were positive for the\n            <jats:italic>ctx<\/jats:italic>\n            A,\n            <jats:italic>zot<\/jats:italic>\n            , and\n            <jats:italic>ace<\/jats:italic>\n            genes. Also, O1 strain VO3 was negative for the\n            <jats:italic>zot<\/jats:italic>\n            gene. All of the non-O1, non-O139 strains were negative for the\n            <jats:italic>ctx<\/jats:italic>\n            A,\n            <jats:italic>zot<\/jats:italic>\n            ,\n            <jats:italic>ace<\/jats:italic>\n            ,\n            <jats:italic>tcpA<\/jats:italic>\n            , and\n            <jats:italic>tcp<\/jats:italic>\n            I genes, and all of the non-O1, non-O139 strains except strain VO26 were negative for\n            <jats:italic>omp<\/jats:italic>\n            U. All of the strains except non-O1, non-O139 strain VO22 were PCR positive for the gene encoding the central regulatory protein,\n            <jats:italic>tox<\/jats:italic>\n            R. All\n            <jats:italic>V. cholerae<\/jats:italic>\n            strains were negative for the NAG-specific\n            <jats:italic>st<\/jats:italic>\n            gene. Of the nine non-\n            <jats:italic>ctx<\/jats:italic>\n            -producing strains of\n            <jats:italic>V. cholerae<\/jats:italic>\n            , only one, non-O1, non-O139 strain VO24, caused fluid accumulation in the rabbit ileal loop assay. The other eight strains, including an O1 strain, an O139 strain, and six non-O1, non-O139 strains, regardless of the source of isolation, caused fluid accumulation after two to five serial passages through the rabbit gut. Culture filtrates of all non-cholera-toxigenic strains grown in AKI media also caused fluid accumulation, suggesting that a new toxin was produced in AKI medium by these strains. Studies of clonality performed by using enterobacterial repetitive intergenic consensus sequence PCR, Box element PCR, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE) collectively indicated that the\n            <jats:italic>V. cholerae<\/jats:italic>\n            O1 and O139 strains had a clonal origin, whereas the non-O1, non-O139 strains belonged to different clones. The clinical isolates closely resembled environmental isolates in their genomic patterns. Overall, there was an excellent correlation among the results of the PCR, AFLP, and PFGE analyses, and individual strains derived from clinical and environmental sources produced similar fingerprint patterns. From the results of this study, we concluded that the non-cholera-toxin-producing strains of\n            <jats:italic>V. cholerae<\/jats:italic>\n            , whether of clinical or environmental origin, possess the ability to produce a new secretogenic toxin that is entirely different from the toxin produced by toxigenic\n            <jats:italic>V. cholerae<\/jats:italic>\n            O1 and O139 strains. We also concluded that the aquatic environment is a reservoir for\n            <jats:italic>V. cholerae<\/jats:italic>\n            O1, O139, non-O1, and non-O139 serogroup strains.\n          <\/jats:p>","DOI":"10.1128\/aem.67.2.910-921.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"910-921","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":198,"title":["Molecular Analysis of\n            <i>Vibrio cholerae<\/i>\n            O1, O139, non-O1, and non-O139 Strains: Clonal Relationships between Clinical and Environmental Isolates"],"prefix":"10.1128","volume":"67","author":[{"given":"D. V.","family":"Singh","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Rajiv Gandhi Centre for Biotechnology, Jagathy, Thiruvananthapuram 695 014, India1;"},{"name":"<!--label omitted: 2-->Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria H.","family":"Matte","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022;"},{"name":"<!--label omitted: 3-->School of Public Health, University of Sa\u0303o Paulo, Sa\u0303o Paulo, SP, 01246-904, Sa\u0303o Paulo, Brazil3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. R.","family":"Matte","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022;"},{"name":"<!--label omitted: 3-->School of Public Health, University of Sa\u0303o Paulo, Sa\u0303o Paulo, SP, 01246-904, Sa\u0303o Paulo, Brazil3;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunny","family":"Jiang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022;"},{"name":"<!--label omitted: 4-->Department of Environmental Analysis and Design, University of California, Irvine, California 926974"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Sabeena","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Rajiv Gandhi Centre for Biotechnology, Jagathy, Thiruvananthapuram 695 014, India1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. N.","family":"Shukla","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India5; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. C.","family":"Sanyal","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India5; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Huq","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022;"},{"name":"<!--label omitted: 6-->Department of Cell and Molecular Biology, University of Maryland, College Park, Maryland 207426;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. R.","family":"Colwell","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212022;"},{"name":"<!--label omitted: 6-->Department of Cell and Molecular Biology, University of Maryland, College Park, Maryland 207426;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90481-U"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(91)90247-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.52.1.45-49.1986"},{"key":"e_1_3_2_5_2","unstructured":"Ausubel\nF. M.\nBrent\nR.\nKingston\nR. E.\nMoore\nD. D.\nSeidman\nJ. G.\nSmith\nJ. A.\nStruhl\nK.\nShort protocols in molecular biology\n3rd ed.\n1995\nJohn Wiley and Sons Inc.\nNew York N.Y"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.5.1315-1317.1993"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.13.1.126-129.1981"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb06993.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.34.100180.002013"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1139\/m69-073"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92811-7"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.114-118.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.910135"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"Colwell\nR. R.\nHuq\nA.\nVibrios in the environment: viable but non-culturable Vibrio cholerae Vibrio cholerae and cholera\u2014molecular to global perspectives.\nWachsmuth\nI. K.\nOlsvik\n\u00d8.\nBlake\nP. A.\nOlsvik\nR.\n1994\n117\n133\nAmerican Society for Microbiology\nWashington D.C.","DOI":"10.1128\/9781555818364.ch9"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb19852.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.41.2.555-558.1981"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.10.2715-2722.1995"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700660228"},{"key":"e_1_3_2_19_2","first-page":"97","article-title":"A non-hemolytic vibrio.","volume":"15","author":"de Moore C. E.","year":"1963","unstructured":"de Moore C. E. A non-hemolytic vibrio. Trop. Geogr. Med. 15 1963 97 107","journal-title":"Trop. Geogr. Med."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1301-1314.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5819-5825.1998"},{"key":"e_1_3_2_22_2","first-page":"865","article-title":"Environmental aspects of cholera epidemiology. II. Occurrence and survival of V. cholerae in the environment.","volume":"78","author":"Feachem R.","year":"1981","unstructured":"Feachem R. Miller C. Drasar B. Environmental aspects of cholera epidemiology. II. Occurrence and survival of V. cholerae in the environment. Trop. Dis. Bull. 78 1981 865 880","journal-title":"Trop. Dis. Bull."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.8.2118-2121.1992"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-112-28043"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1994.tb00114.x"},{"key":"e_1_3_2_26_2","first-page":"324","article-title":"The fate of enteric bacteria in estuarine and marine environment.","volume":"3","author":"Grimes D. J.","year":"1986","unstructured":"Grimes D. J. Atwell R. A. Brayton P. R. Palmer L. M. Rollins D. M. Roszak D. B. Singleton F. L. Tamplin M. L. Colwell R. R. The fate of enteric bacteria in estuarine and marine environment. Microb. Sci. 3 1986 324 329","journal-title":"Microb. Sci."},{"key":"e_1_3_2_27_2","first-page":"781","article-title":"Investigation into the nature of Vibrio cholerae strains isolated from the epidemic of gastroenteritis in Kumph fair at Allahabad in 1956.","volume":"10","author":"Gupta N. P.","year":"1956","unstructured":"Gupta N. P. Gupta S. P. Manglik V. S. Prasad B. G. Yajnik B. S. Investigation into the nature of Vibrio cholerae strains isolated from the epidemic of gastroenteritis in Kumph fair at Allahabad in 1956. Indian J. Med Sci. 10 1956 781 791","journal-title":"Indian J. Med Sci."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.9.3859-3863.1994"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.36.1.209-214.1982"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.4.1487"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.41.2.559-560.1981"},{"key":"e_1_3_2_32_2","first-page":"198","article-title":"Vibrios in the marine and estuarine environment: tracking of Vibrio cholerae.","volume":"2","author":"Huq A.","year":"1996","unstructured":"Huq A. Colwell R. R. Vibrios in the marine and estuarine environment: tracking of Vibrio cholerae. J. Ecosyst. Health 2 1996 198 214","journal-title":"J. Ecosyst. Health"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)92038-2"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2370-2373.1990"},{"key":"e_1_3_2_35_2","first-page":"86","article-title":"Comparison of Vibrio cholerae serotype O1 isolated from patient and aquatic environment.","volume":"96","author":"Huq A.","year":"1993","unstructured":"Huq A. Parveen S. Qadri F. Colwell R. R. Comparison of Vibrio cholerae serotype O1 isolated from patient and aquatic environment. J. Trop. Med. Hyg. 96 1993 86 92","journal-title":"J. Trop. Med. Hyg."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.5.1090-1093.1987"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(90)90345-F"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.5.1684-1686.1994"},{"key":"e_1_3_2_39_2","first-page":"87","article-title":"The aquatic flora and fauna as reservoir of Vibrio cholerae: a review.","volume":"12","author":"Islam M. S.","year":"1994","unstructured":"Islam M. S. Drasar B. S. Sack R. B. The aquatic flora and fauna as reservoir of Vibrio cholerae: a review. J. Diarrh. Dis. Res. 12 1994 87 96","journal-title":"J. Diarrh. Dis. Res."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1986.tb03037.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-7-1881"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.1.148-153.2000"},{"key":"e_1_3_2_43_2","unstructured":"Kamal\nA. M.\nThe seventh pandemic of cholera\nCholera.\nBarua\nD.\nBurros\nW.\n1974\n1\n14\nThe W. B. Saunders Company\nPhiladelphia Pa"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.11.3191-3198.1995"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/20715"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(95)90076-4"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.1.161-167.1988"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.4.1105-1112.1996"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.13.3479"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)91036-0"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2537530"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1139\/m96-014"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.10.2537-2543.1996"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(76)90915-6"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90006-0"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90480-5"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.8.2898-2904.1995"},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","unstructured":"Rodrigue\nD. C.\nPopovic\nT.\nWachsmuth\nI. K.\nNon-toxigenic Vibrio cholerae O1 infection in the Unites States\nVibrio cholerae and cholera\u2014molecular to global perspective.\nWachsmuth\nI. K.\nBlake\nP. A.\nOlsvik\nR.\n1994\n69\n76\nAmerican Society for Microbiology\nWashington D.C.","DOI":"10.1128\/9781555818364.ch5"},{"key":"e_1_3_2_59_2","first-page":"553","article-title":"Hemolytic and non-hemolytic El Tor vibrios.","volume":"23","author":"Roy S.","year":"1963","unstructured":"Roy S. Mukerjee S. Tanamal S. J. W. Hemolytic and non-hemolytic El Tor vibrios. Ann. Biochem. Exp. Med. 23 1963 553 558","journal-title":"Ann. Biochem. Exp. Med."},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.8.3101-3108.1996"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.5.1114-1117.1996"},{"key":"e_1_3_2_62_2","first-page":"207","article-title":"Vibrio toxin","volume":"119","author":"Sanyal S. C.","year":"1986","unstructured":"Sanyal S. C. Vibrio toxin Pharmacology of bacterial toxins. International encyclopedia of pharmacology and therapeutics Dorner F. Drews J. 119 1986 207 225 Pergamon Press Oxford, United Kingdom","journal-title":"Pharmacology of bacterial toxins. International encyclopedia of pharmacology and therapeutics"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)92449-2"},{"key":"e_1_3_2_64_2","first-page":"3","article-title":"A new enterotoxin produced by V. cholerae O1.","volume":"2","author":"Sanyal S. C.","year":"1984","unstructured":"Sanyal S. C. Neogi P. K. B. Alam K. Huq M. I. Al-Mahmud K. A. A new enterotoxin produced by V. cholerae O1. J. Diarrh. Dis. Res. 2 1984 3 12","journal-title":"J. Diarrh. Dis. Res."},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.3.756-763.1998"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1998.tb02357.x"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1995.tb00182.x"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-45-1-31"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-45-1-35"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)55030-3"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.37.4.750-759.1979"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-40-4-246"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.6.1591-1598.1989"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-5-398"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.21.4407"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5270.1910"},{"key":"e_1_3_2_78_2","unstructured":"World Health Organization\nManual for laboratory investigation of acute enteric infections. Publication WHO CDD\/83.3.\n1987\nProgram for Control of Diarrhoeal Diseases World Health Organization\nGeneva Switzerland"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.45.1.192-196.1984"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1985.tb00861.x"}],"updated-by":[{"DOI":"10.1128\/aem.67.7.3331-3331.2001","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000}}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.67.2.910-921.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.67.2.910-921.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T11:30:16Z","timestamp":1645615816000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.67.2.910-921.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":79,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["10.1128\/AEM.67.2.910-921.2001"],"URL":"https:\/\/doi.org\/10.1128\/aem.67.2.910-921.2001","relation":{"correction":[{"id-type":"doi","id":"10.1128\/AEM.67.7.3331-3331.2001","asserted-by":"object"}]},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]},"assertion":[{"value":"2000-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}