{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:13:57Z","timestamp":1790507637438,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Because\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            oocysts are very resistant to conventional water treatment processes, including chemical disinfection, we determined the kinetics and extent of their inactivation by monochromatic, low-pressure (LP), mercury vapor lamp UV radiation and their subsequent potential for DNA repair of UV damage. A UV collimated-beam apparatus was used to expose suspensions of purified\n            <jats:italic>C. parvum<\/jats:italic>\n            oocysts in phosphate-buffered saline, pH 7.3, at 25\u00b0C to various doses of monochromatic LP UV.\n            <jats:italic>C. parvum<\/jats:italic>\n            infectivity reductions were rapid, approximately first order, and at a dose of 3 mJ\/cm\n            <jats:sup>2<\/jats:sup>\n            (=30 J\/m\n            <jats:sup>2<\/jats:sup>\n            ), the reduction reached the cell culture assay detection limit of \u223c3 log\n            <jats:sub>10<\/jats:sub>\n            . At UV doses of 1.2 and 3 mJ\/cm\n            <jats:sup>2<\/jats:sup>\n            , the log\n            <jats:sub>10<\/jats:sub>\n            reductions of\n            <jats:italic>C. parvum<\/jats:italic>\n            oocyst infectivity were not significantly different for control oocysts and those exposed to dark or light repair conditions for UV-induced DNA damage. These results indicate that\n            <jats:italic>C. parvum<\/jats:italic>\n            oocysts are very sensitive to inactivation by low doses of monochromatic LP UV radiation and that there is no phenotypic evidence of either light or dark repair of UV-induced DNA damage.\n          <\/jats:p>","DOI":"10.1128\/aem.67.7.3029-3032.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"3029-3032","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":135,"title":["Low-Pressure UV Inactivation and DNA Repair Potential of\n            <i>Cryptosporidium parvum<\/i>\n            Oocysts"],"prefix":"10.1128","volume":"67","author":[{"given":"Gwy-Am","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-74001;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl G.","family":"Linden","sequence":"additional","affiliation":[{"name":"Department of Civil &amp; Environmental Engineering, Duke University, Durham, North Carolina 277082; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Arrowood","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Sobsey","sequence":"additional","affiliation":[{"name":"Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-74001;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282084"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2307\/3283822"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1996.tb05015.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.1999.tb08602.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(95)00072-S"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.2000.tb09008.x"},{"key":"e_1_3_2_8_2","unstructured":"Clark\nR. 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