{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T21:20:21Z","timestamp":1777584021391,"version":"3.51.4"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Detection of biological weapons is a primary concern in force protection, treaty verification, and safeguarding civilian populations against domestic terrorism. One great concern is the detection of\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            , the causative agent of anthrax. Assays for detection in the laboratory often employ inactivated preparations of spores or nonpathogenic simulants. This study uses several common biodetection platforms to detect\n            <jats:italic>B. anthracis<\/jats:italic>\n            spores that have been inactivated by two methods and compares those data to detection of spores that have not been inactivated. The data demonstrate that inactivation methods can affect the sensitivity of nucleic acid- and antibody-based assays for the detection of\n            <jats:italic>B. anthracis<\/jats:italic>\n            spores. These effects should be taken into consideration when comparing laboratory results to data collected and assayed during field deployment.\n          <\/jats:p>","DOI":"10.1128\/aem.67.8.3665-3670.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"3665-3670","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["<i>Bacillus<\/i>\n            Spore Inactivation Methods Affect Detection Assays"],"prefix":"10.1128","volume":"67","author":[{"given":"Jessica L.","family":"Dang","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Geo-Centers, Inc., Lanham, Maryland 207061;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Heroux","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Geo-Centers, Inc., Lanham, Maryland 207061;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Kearney","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 352942; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ameneh","family":"Arasteh","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Research and Technology Directorate, U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland 210103"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Gostomski","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Research and Technology Directorate, U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland 210103"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"Emanuel","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Research and Technology Directorate, U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Maryland 210103"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0944-5013(11)80054-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7538699"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.49.2.291-297.1985"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3101672.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.986"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/09593339109385046"},{"key":"e_1_3_2_8_2","first-page":"1","article-title":"Pathogenicity islands and the evolution of bacterial pathogens.","volume":"5","author":"Lee C. 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