{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:52:10Z","timestamp":1760345530092},"reference-count":19,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Ralstonia eutropha<\/jats:italic>\n            KT1, which degrades trichloroethylene, was injected into the aquifer after activation with toluene, and then the number of bacteria was monitored by in situ PCR targeting the phenol hydroxylase gene and by fluorescent in situ hybridization (FISH) targeting 16S rRNA. Before injection of the bacterial suspension, the total concentration of bacteria in the groundwater was approximately 3 \u00d7 10\n            <jats:sup>5<\/jats:sup>\n            cells\/ml and the amount of\n            <jats:italic>Ralstonia<\/jats:italic>\n            and bacteria carrying the phenol hydroxylase gene as a percentage of total bacterial cells was less than 0.1%. The concentration of bacteria carrying the phenol hydroxylase gene detected by in situ PCR was approximately 3 \u00d7 10\n            <jats:sup>7<\/jats:sup>\n            cells\/ml 1 h after injection, and the concentration of\n            <jats:italic>Ralstonia<\/jats:italic>\n            detected by FISH was similar. The number of bacteria detected by in situ PCR was similar to that detected by FISH 4 days after the start of the extraction of groundwater. On and after day 7, however, the number of bacterial cells detected by FISH was less than that detected by in situ PCR.\n          <\/jats:p>","DOI":"10.1128\/aem.68.1.412-416.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:56:57Z","timestamp":1027763817000},"page":"412-416","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Monitoring of\n            <i>Ralstonia eutropha<\/i>\n            KT1 in Groundwater in an Experimental Bioaugmentation Field by In Situ PCR"],"prefix":"10.1128","volume":"68","author":[{"given":"Katsuji","family":"Tani","sequence":"first","affiliation":[{"name":"Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Muneta","sequence":"additional","affiliation":[{"name":"Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanji","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Corporate Research and Development Center, Kurita Water Industries Ltd., Morinosato, Atsugi, Kanagawa 243-0124"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsutoshi","family":"Shibuya","sequence":"additional","affiliation":[{"name":"Institute of Technology, Simizu Corporation, Kohtoh, Tokyo 135-8530, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Nasu","sequence":"additional","affiliation":[{"name":"Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.12.4907-4913.1997"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09029.x"},{"key":"e_1_3_2_4_2","unstructured":"Nonculturable microorganisms in the environment 2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0922-338X(99)80003-8"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.11.4074-4082.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2000.tb00707.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb10410.x"},{"key":"e_1_3_2_9_2","first-page":"415","volume":"25","year":"1979","unstructured":"Kogure, K., U. 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