{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:12:25Z","timestamp":1762099945301},"reference-count":29,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Water quality assessment involves the specific, sensitive, and rapid detection of bacterial indicators and pathogens in water samples, including viable but nonculturable (VBNC) cells. This work evaluates the specificity and sensitivity of a new method which combines a fluorescent in situ hybridization (FISH) approach with a physiological assay (direct viable count [DVC]) for the direct enumeration, at the single-cell level, of highly diluted viable cells of members of the family\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            in freshwater and drinking water after membrane filtration. The approach (DVC-FISH) uses a new direct detection device, the laser scanning cytometer (Scan RDI). Combining the DVC-FISH method on a membrane with Scan RDI detection makes it possible to detect as few as one targeted cell in approximately 10\n            <jats:sup>8<\/jats:sup>\n            nontargeted cells spread over the membrane. The ability of this new approach to detect and enumerate VBNC enterobacterial cells in freshwater and drinking water distribution systems was investigated and is discussed.\n          <\/jats:p>","DOI":"10.1128\/aem.68.10.5057-5063.2002","type":"journal-article","created":{"date-parts":[[2002,9,25]],"date-time":"2002-09-25T18:02:42Z","timestamp":1032976962000},"page":"5057-5063","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Rapid and Sensitive Enumeration of Viable Diluted Cells of Members of the Family\n            <i>Enterobacteriaceae<\/i>\n            in Freshwater and Drinking Water"],"prefix":"10.1128","volume":"68","author":[{"given":"Julia","family":"Baudart","sequence":"first","affiliation":[{"name":"NSERC Industrial Chair on Drinking Water, Civil, Geological and Mining Engineering, E\u0301cole Polytechnique de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada H3C 3A7"},{"name":"US Filter-John Meunier, St. Laurent, Que\u0301bec, Canada H4S 2B3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301e","family":"Coallier","sequence":"additional","affiliation":[{"name":"NSERC Industrial Chair on Drinking Water, Civil, Geological and Mining Engineering, E\u0301cole Polytechnique de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada H3C 3A7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Laurent","sequence":"additional","affiliation":[{"name":"NSERC Industrial Chair on Drinking Water, Civil, Geological and Mining Engineering, E\u0301cole Polytechnique de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada H3C 3A7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miche\u0300le","family":"Pre\u0301vost","sequence":"additional","affiliation":[{"name":"US Filter-John Meunier, St. Laurent, Que\u0301bec, Canada H4S 2B3"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2000.tb00557.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.9.3007-3011.1992"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00048-2"},{"key":"e_1_3_2_6_2","first-page":"18","volume":"4","year":"1996","unstructured":"Brailsford, M. 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