{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:38:51Z","timestamp":1784893131486,"version":"3.55.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Some strains of\n            <jats:italic>Bacillus sphaericus<\/jats:italic>\n            are entomopathogenic to mosquito larvae, which transmit diseases, such as filariasis and malaria, affecting millions of people worldwide. This species is unable to use hexoses and pentoses as unique carbon sources, which was proposed to be due to the lack of glycolytic enzymes, such as 6-phosphofructokinase (PFK). In this study, PFK activity was detected and the\n            <jats:italic>pfk<\/jats:italic>\n            gene was cloned and sequenced. Furthermore, this gene was shown to be present in strains belonging to all the homology groups of this heterogeneous species, in which PFK activity was also detected. A careful sequence analysis revealed the conservation of different catalytic and regulatory residues, as well as the enzyme's phylogenetic affiliation with the family of allosteric ATP-PFK enzymes.\n          <\/jats:p>","DOI":"10.1128\/aem.68.12.6410-6415.2002","type":"journal-article","created":{"date-parts":[[2002,11,26]],"date-time":"2002-11-26T00:04:48Z","timestamp":1038269088000},"page":"6410-6415","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Existence of a True Phosphofructokinase in\n            <i>Bacillus sphaericus<\/i>\n            : Cloning and Sequencing of the\n            <i>pfk<\/i>\n            Gene"],"prefix":"10.1128","volume":"68","author":[{"given":"Alejandro F.","family":"Alice","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiologi\u0301a, Departamento de Qui\u0301mica Biolo\u0301gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaspar","family":"Pe\u0301rez-Marti\u0301nez","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnologi\u0301a, Instituto de Agroqui\u0301mica y Tecnologi\u0301a de Alimentos, CSIC, Paterna, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Sa\u0301nchez-Rivas","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiologi\u0301a, Departamento de Qui\u0301mica Biolo\u0301gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-2-367"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80014-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.3.425-436.1991"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.41.010196.002315"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"Couch T. L. 2000. Industrial fermentation and formulation of entomopathogenic bacteria p. 297-316. In J.-F. Charles et al. (ed.) Entomopathogenic bacteria: from laboratory to field application. Kluwer Academic Publishers Dordrecht The Netherlands.","DOI":"10.1007\/978-94-017-1429-7_16"},{"key":"e_1_3_2_7_2","unstructured":"Cutting S. M. and P. B. Vander Horn. 1990. Genetic analysis p. 27-74. In C. R. Harwood and S. M. Cutting (ed.) Molecular biological methods for Bacillus . J. Wiley and Sons Ltd. Chichester United Kingdom."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80019-9"},{"key":"e_1_3_2_9_2","first-page":"164","volume":"5","year":"1989","unstructured":"Felsenstein, J. 1989. Phylip: phylogeny inference package (version 3.2). 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Nucleic Acids Res.21:4317-4331.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01548.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.15.3584"},{"key":"e_1_3_2_27_2","first-page":"315","volume":"3","year":"1984","unstructured":"Yousten, A. A. 1984. Bacillus sphaericus: microbiological factors related to its potential as a mosquito larvicide. Adv. Biotechnol. Processes3:315-343.","journal-title":"Adv. Biotechnol. 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