{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:09:00Z","timestamp":1767924540551,"version":"3.49.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Identification of\n            <jats:italic>Bifidobacterium lactis<\/jats:italic>\n            and\n            <jats:italic>Bifidobacterium animalis<\/jats:italic>\n            is problematic because of phenotypic and genetic homogeneities and has raised the question of whether they belong to one unique taxon. Analysis of the 16S-23S internally transcribed spacer region of\n            <jats:italic>B. lactis<\/jats:italic>\n            DSM10140\n            <jats:sup>T<\/jats:sup>\n            ,\n            <jats:italic>B. animalis<\/jats:italic>\n            ATCC 25527\n            <jats:sup>T<\/jats:sup>\n            , and six potential\n            <jats:italic>B. lactis<\/jats:italic>\n            strains suggested two distinct clusters. Two specific 16S-23S spacer rRNA gene-targeted primers have been developed for specific detection of\n            <jats:italic>B. animalis<\/jats:italic>\n            . All of the molecular techniques used (\n            <jats:italic>B. lactis<\/jats:italic>\n            or\n            <jats:italic>B. animalis<\/jats:italic>\n            PCR primers, enterobacterial repetitive intergenic consensus PCR) demonstrated that\n            <jats:italic>B. lactis<\/jats:italic>\n            and\n            <jats:italic>B. animalis<\/jats:italic>\n            form two main groups and suggest a revision of the strains assigned to\n            <jats:italic>B. animalis<\/jats:italic>\n            . We propose that\n            <jats:italic>B. lactis<\/jats:italic>\n            should be separated from\n            <jats:italic>B. animalis<\/jats:italic>\n            at the subspecies level.\n          <\/jats:p>","DOI":"10.1128\/aem.68.12.6429-6434.2002","type":"journal-article","created":{"date-parts":[[2002,11,26]],"date-time":"2002-11-26T00:04:48Z","timestamp":1038269088000},"page":"6429-6434","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Rapid Identification, Differentiation, and Proposed New Taxonomic Classification of\n            <i>Bifidobacterium lactis<\/i>"],"prefix":"10.1128","volume":"68","author":[{"given":"Marco","family":"Ventura","sequence":"first","affiliation":[{"name":"Nestle\u0301 Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Zink","sequence":"additional","affiliation":[{"name":"Nestle\u0301 Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-1-102"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2000.tb02568.x"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.5.2202-2208.1999"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1618-1621.2001"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-5-1633"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.4.1268-1273.1997"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.10.3668-3672.1996"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(97)80048-3"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.8.2144-2151.1998"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-6946(99)00024-2"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-40-4-323"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(95)00013-5"},{"key":"e_1_3_1_14_2","first-page":"17","volume":"191","year":"2001","unstructured":"Roy, D., and S. Sirois. 2001. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of ldh gene. FEMS Microbiol. Lett.191:17-24.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-1-259"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010355003674"},{"key":"e_1_3_1_17_2","unstructured":"Staley J. T. and N. R. Krieg. 1986. Classification of procaryotic organisms: an overview p. 1-4. In P. H. A. Sneath N. S. Mair M. E. Sharpe and J. G. Holt (ed.) Bergey's manual of systematic bacteriology vol. 1. The Williams & Wilkins Co. 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