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The immunogenic<jats:italic>B. abortus<\/jats:italic>ribosomal protein L7\/L12 is a promising candidate antigen for the development of oral live vaccines against brucellosis, using food-grade lactic acid bacteria (LAB) as a carrier. The<jats:italic>L7\/L12<\/jats:italic>gene was expressed in<jats:italic>Lactococcus lactis<\/jats:italic>, the model LAB, under the nisin-inducible promoter. Using different signals, L7\/L12 was produced in cytoplasmic, cell-wall-anchored, and secreted forms. Cytoplasmic production of L7\/L12 gave a low yield, estimated at 0.5 mg\/liter. Interestingly, a secretable form of this normally cytoplasmic protein via fusion with a signal peptide resulted in increased yield of L7\/L12 to 3 mg\/liter; secretion efficiency (SE) was 35%. A fusion between the mature moiety of the staphylococcal nuclease (Nuc) and L7\/L12 further increased yield to 8 mg\/liter. Fusion with a synthetic propeptide (LEISSTCDA) previously described as an enhancer for heterologous protein secretion in<jats:italic>L. lactis<\/jats:italic>(Y. Le Loir, A. Gruss, S. D. Ehrlich, and P. Langella, J. Bacteriol. 180:1895-1903, 1998) raised the yield to 8 mg\/liter and SE to 50%. A surface-anchored L7\/L12 form in<jats:italic>L. lactis<\/jats:italic>was obtained by fusing the cell wall anchor of<jats:italic>Streptococcus pyogenes<\/jats:italic>M6 protein to the C-terminal end of L7\/L12. The fusions described allow the production and targeting of L7\/L12 in three different locations in<jats:italic>L. lactis<\/jats:italic>. This is the first example of a<jats:italic>B. abortus<\/jats:italic>antigen produced in a food-grade bacterium and opens new perspectives for alternative vaccine strategies against brucellosis.<\/jats:p>","DOI":"10.1128\/aem.68.2.910-916.2002","type":"journal-article","created":{"date-parts":[[2003,2,26]],"date-time":"2003-02-26T10:22:46Z","timestamp":1046254966000},"page":"910-916","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":119,"title":["Production and Targeting of the<i>Brucella abortus<\/i>Antigen L7\/L12 in<i>Lactococcus lactis<\/i>: a First Step towards Food-Grade Live Vaccines against Brucellosis"],"prefix":"10.1128","volume":"68","author":[{"given":"Luciana A.","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Appliqu\u00e9e, Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"},{"name":"Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasco","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Le Loir","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Appliqu\u00e9e, Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakhya","family":"Dieye","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Appliqu\u00e9e, Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Piard","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Appliqu\u00e9e, Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gruss","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Appliqu\u00e9e, Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Langella","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Appliqu\u00e9e, Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00231-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.917-922.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00165-X"},{"key":"e_1_3_2_5_2","first-page":"83","year":"1997","unstructured":"Chamberlain, L., J. 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