{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:23:35Z","timestamp":1791127415286,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Partial sequence analysis of the ribosomal operon in\n            <jats:italic>Xanthomonas axonopodis<\/jats:italic>\n            allowed discrimination among strains causing the A, B, and C types of citrus bacterial canker (CBC) and quantification of the relationship of these organisms with other species and pathovars in the same genus. Sets of primers based on sequence differences in the internally transcribed spacer and on a sequence from the plasmid gene\n            <jats:italic>pthA<\/jats:italic>\n            involved in virulence were designed for specific identification of xanthomonads causing CBC diseases. The two sets were validated with a collection of\n            <jats:italic>Xanthomonas<\/jats:italic>\n            strains associated with citrus species. The primer set based on ribosomal sequences had a high level of specificity for\n            <jats:italic>X. axonopodis<\/jats:italic>\n            pv. citri, whereas the set based on the\n            <jats:italic>pthA<\/jats:italic>\n            gene was universal for all types of CBC organisms. Moreover, the relationships among worldwide\n            <jats:italic>Xanthomonas<\/jats:italic>\n            strains causing CBC were analyzed by amplification of repetitive sequences (enterobacterial repetitive intergenic consensus and BOX elements). Under specific conditions, pathotypes of these\n            <jats:italic>Xanthomonas<\/jats:italic>\n            strains could be discerned, and subgroups of the pathotypes were identified. Subgroups of strains were associated with certain geographic areas of the world, and on this basis the origin of type A strains introduced into Florida could be inferred.\n          <\/jats:p>","DOI":"10.1128\/aem.68.3.1257-1264.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"1257-1264","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":135,"title":["Genetic Relationship among Worldwide Strains of\n            <i>Xanthomonas<\/i>\n            Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR"],"prefix":"10.1128","volume":"68","author":[{"given":"J.","family":"Cubero","sequence":"first","affiliation":[{"name":"Citrus Research and Education Center, University of Florida, Lake Alfred, Florida 33850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. H.","family":"Graham","sequence":"additional","affiliation":[{"name":"Citrus Research and Education Center, University of Florida, Lake Alfred, Florida 33850"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"127","volume":"37","year":"1984","unstructured":"Civerolo, E. 1984. Bacterial canker disease of citrus. J. Rio Grande Val. Hortic. Soc.37:127-145.","journal-title":"J. Rio Grande Val. Hortic. Soc."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.6.2849-2852.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2307\/1932409"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.9.2724-2730.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-80-829"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1094\/PD-76-0889"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","unstructured":"Hartung J. S. and E. L. Civerolo. 1989. Restriction fragment length polymorphisms distinguish Xanthomonas campestris strains isolated from Florida citrus nurseries from Xanthomonas campestris pv. citri p. 503-508. In Proceedings of the 7th International Conference on Plant Pathogenic Bacteria. Akademiai Kiado Budapest Hungary.","DOI":"10.1094\/Phyto-79-793"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.4.1143-1148.1993"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-86-95"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-328"},{"key":"e_1_3_2_12_2","first-page":"21","year":"1969","unstructured":"Jukes, T. H., and C. R. Cantor. 1969. Evolution of protein molecules, p. 21-132. In H. N. Munro (ed.), Mammalian protein metabolism. 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Evol.4:406-425.","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS.2001.85.4.340"},{"key":"e_1_3_2_23_2","first-page":"802","volume":"81","year":"1991","unstructured":"Swarup, S., R. De Feyter, R. H. Brlansky, and D. Gabriel. 1991. A pathogenicity locus from Xanthomonas citri enables strains from several pathovars of X. campestris to elicit canker lesions in citrus. Mol. Plant Pathol.81:802-809.","journal-title":"Mol. Plant Pathol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2000.90.7.677"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008676508688"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-8-0627"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.68.3.1257-1264.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.68.3.1257-1264.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T12:47:57Z","timestamp":1645620477000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.68.3.1257-1264.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["10.1128\/AEM.68.3.1257-1264.2002"],"URL":"https:\/\/doi.org\/10.1128\/aem.68.3.1257-1264.2002","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,3]]},"assertion":[{"value":"2001-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}