{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:34:06Z","timestamp":1759638846177},"reference-count":52,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Genomic DNAs from human\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            isolates previously typed by analysis of the 18S ribosomal DNA locus (\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            bovine genotype,\n            <jats:italic>C. parvum<\/jats:italic>\n            human genotype,\n            <jats:italic>Cryptosporidium meleagridis<\/jats:italic>\n            , and\n            <jats:italic>Cryptosporidium felis<\/jats:italic>\n            ) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous\n            <jats:italic>Cryptosporidium wrairi<\/jats:italic>\n            sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species and\n            <jats:italic>C. parvum<\/jats:italic>\n            genotypes.\n          <\/jats:p>","DOI":"10.1128\/aem.68.4.2071-2076.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"2071-2076","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["PCR-Restriction Fragment Length Polymorphism Analysis of a Diagnostic 452-Base-Pair DNA Fragment Discriminates between\n            <i>Cryptosporidium parvum<\/i>\n            and\n            <i>C. meleagridis<\/i>\n            and between\n            <i>C. parvum<\/i>\n            Isolates of Human and Animal Origin"],"prefix":"10.1128","volume":"68","author":[{"given":"K.","family":"Guyot","sequence":"first","affiliation":[{"name":"Ecologie du Parasitisme, IFR 17, Institut Pasteur de Lille, 59019 Lille"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Follet-Dumoulin","sequence":"additional","affiliation":[{"name":"Ecologie du Parasitisme, IFR 17, Institut Pasteur de Lille, 59019 Lille"},{"name":"Laboratoire Environnement et Sante\u0301, Universite\u0301 Catholique de Lille, 59046 Lille"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Recourt","sequence":"additional","affiliation":[{"name":"Ecologie du Parasitisme, IFR 17, Institut Pasteur de Lille, 59019 Lille"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Lelie\u0300vre","sequence":"additional","affiliation":[{"name":"Ecologie du Parasitisme, IFR 17, Institut Pasteur de Lille, 59019 Lille"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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