{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T07:25:48Z","timestamp":1771485948386,"version":"3.50.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this study, enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were optimized for characterization of\n            <jats:italic>Arcobacter butzleri<\/jats:italic>\n            ,\n            <jats:italic>Arcobacter cryaerophilus<\/jats:italic>\n            , and\n            <jats:italic>Arcobacter skirrowii<\/jats:italic>\n            . In addition, a simple and rapid DNA extraction method was tested for use in both typing procedures. Both methods had satisfactory typeability and discriminatory power, but the fingerprints generated with ERIC-PCR were more reproducible and complex than those obtained with RAPD-PCR. The use of nondiluted boiled cell suspensions as DNA templates was found to be very useful in ERIC-PCR. Characterization of large numbers of\n            <jats:italic>Arcobacter<\/jats:italic>\n            isolates is therefore preferably performed by the ERIC-PCR procedure. Isolates for which almost identical ERIC fingerprints are generated may subsequently be characterized by RAPD-PCR, although adjustment and standardization of the amount of the DNA template are necessary. In the second part of this study, the genotypic diversity of arcobacters present on broiler carcasses was assessed by using both typing methods. A total of 228 cultures from 24 samples were examined after direct isolation and enrichment. The isolates were identified by using a multiplex PCR as\n            <jats:italic>A. butzleri<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 182) and\n            <jats:italic>A. cryaerophilus<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 46). A total of 131 types (91\n            <jats:italic>A. butzleri<\/jats:italic>\n            types and 40\n            <jats:italic>A. cryaerophilus<\/jats:italic>\n            types) were discerned without discordance between the two typing techniques. The analysis of the poultry isolates showed that poultry products may harbor not only more than one species but also multiple genotypes. All genotypes were confined to one poultry sample, and only three genotypes were found after simultaneous enrichment and direct isolation. These results demonstrate that different outcomes can be obtained in epidemiological studies depending on the isolation procedure used and the number of isolates characterized.\n          <\/jats:p>","DOI":"10.1128\/aem.68.5.2172-2178.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"2172-2178","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":128,"title":["Assessment of the Genetic Diversity among Arcobacters Isolated from Poultry Products by Using Two PCR-Based Typing Methods"],"prefix":"10.1128","volume":"68","author":[{"given":"Kurt","family":"Houf","sequence":"first","affiliation":[{"name":"Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lieven","family":"De Zutter","sequence":"additional","affiliation":[{"name":"Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Van Hoof","sequence":"additional","affiliation":[{"name":"Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Vandamme","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1998.00437.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1996.tb00030.x"},{"key":"e_1_3_2_4_2","first-page":"111","volume":"27","year":"1995","unstructured":"Fernandez, H., X. 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