{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:19:51Z","timestamp":1791303591750,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Experimentally inoculated sheep and cattle were used as models of natural ruminant infection to investigate the pattern of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157:H7 shedding and gastrointestinal tract (GIT) location. Eighteen forage-fed cattle were orally inoculated with\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7, and fecal samples were cultured for the bacteria. Three distinct patterns of shedding were observed: 1 month, 1 week, and 2 months or more. Similar patterns were confirmed among 29 forage-fed sheep and four cannulated steers. To identify the GIT location of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7, sheep were sacrificed at weekly intervals postinoculation and tissue and digesta cultures were taken from the rumen, abomasum, duodenum, lower ileum, cecum, ascending colon, descending colon, and rectum.\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 was most prevalent in the lower GIT digesta, specifically the cecum, colon, and feces. The bacteria were only inconsistently cultured from tissue samples and only during the first week postinoculation. These results were supported in studies of four Angus steers with cannulae inserted into both the rumen and duodenum. After the steers were inoculated, ruminal, duodenal, and fecal samples were cultured periodically over the course of the infection. The predominant location of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 persistence was the lower GIT.\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 was rarely cultured from the rumen or duodenum after the first week postinoculation, and this did not predict if animals went on to shed the bacteria for 1 week or 1 month. These findings suggest the colon as the site for\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 persistence and proliferation in mature ruminant animals.\n          <\/jats:p>","DOI":"10.1128\/aem.68.5.2269-2277.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"2269-2277","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":172,"title":["Gastrointestinal Tract Location of\n            <i>Escherichia coli<\/i>\n            O157:H7 in Ruminants"],"prefix":"10.1128","volume":"68","author":[{"given":"Luke J.","family":"Grauke","sequence":"first","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indira T.","family":"Kudva","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang Won","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl W.","family":"Hunt","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Williams","sequence":"additional","affiliation":[{"name":"Division of Statistics, University of Idaho, Moscow, Idaho 83844"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn J.","family":"Hovde","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.12.3013-3017.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.50.1.355"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1993.03500170047032"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.3.726"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)91928-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199508103330608"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.1.27-32.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-63.11.1467"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2000.tb05332.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.11.4926-4934.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1586-1590.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.5.466"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4143-1_16"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.2999"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.5.1519-1525.1996"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.3.1218-1224.2001"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.2959"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036079"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.4.363"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.5.462"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800051633"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268896007212"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.7.3233-3235.1999"},{"key":"e_1_3_2_25_2","unstructured":"Kaper J. 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