{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T10:58:52Z","timestamp":1697194732258},"reference-count":19,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Buchnera strains from most aphid subfamilies studied to date have been found to carry the leucine gene cluster (\n            <jats:italic>leuA<\/jats:italic>\n            , -\n            <jats:italic>B<\/jats:italic>\n            , -\n            <jats:italic>C<\/jats:italic>\n            , and -\n            <jats:italic>D<\/jats:italic>\n            ) on a plasmid, an organization unique among bacteria. Here, however, we demonstrate a classical chromosomal location of the cluster in\n            <jats:italic>Buchnera<\/jats:italic>\n            sp. strain PSY from the aphid\n            <jats:italic>Pemphigus spyrothecae<\/jats:italic>\n            (subfamily Pemphiginae). The genes that flank\n            <jats:italic>leuABCD<\/jats:italic>\n            in\n            <jats:italic>Buchnera<\/jats:italic>\n            sp. strain PSY appear to be adjacent in the genome of\n            <jats:italic>Buchnera<\/jats:italic>\n            sp. strain APS, a strain carrying a leucine plasmid. We propose that the presence of a leucine plasmid predates the diversification of symbiotic\n            <jats:italic>Buchnera<\/jats:italic>\n            and that the chromosomal location observed in\n            <jats:italic>Buchnera<\/jats:italic>\n            sp. strain PSY arose by a transfer of the leucine genes from a plasmid to the chromosome.\n          <\/jats:p>","DOI":"10.1128\/aem.68.5.2572-2575.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"2572-2575","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization of the Leucine Cluster in\n            <i>Buchnera<\/i>\n            sp. Strain PSY, a Primary Endosymbiont of the Aphid\n            <i>Pemphigus spyrothecae<\/i>"],"prefix":"10.1128","volume":"68","author":[{"given":"Beatriz","family":"Sabater-Mun\u0303oz","sequence":"first","affiliation":[{"name":"Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Vale\u0300ncia, Vale\u0300ncia 46071"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Go\u0301mez-Valero","sequence":"additional","affiliation":[{"name":"Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Vale\u0300ncia, Vale\u0300ncia 46071"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roeland C. H. J.","family":"van Ham","sequence":"additional","affiliation":[{"name":"Centro de Astrobiologi\u0301a, INTA-CSIC, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco J.","family":"Silva","sequence":"additional","affiliation":[{"name":"Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Vale\u0300ncia, Vale\u0300ncia 46071"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amparo","family":"Latorre","sequence":"additional","affiliation":[{"name":"Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Vale\u0300ncia, Vale\u0300ncia 46071"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.000415"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006447"},{"key":"e_1_3_2_4_2","first-page":"67","volume":"41","year":"1995","unstructured":"Bracho, A. M., D. Mart\u00ednez-Torres, A. Moya, and A. Latorre. 1995. Discovery and molecular characterization of a plasmid localized in Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi. J. Mol. Evol41:67-73.","journal-title":"J. Mol. Evol"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.43.1.17"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(89)90110-9"},{"key":"e_1_3_2_7_2","first-page":"367","year":"1987","unstructured":"Heie, O. E. 1987. Paleontology and phylogeny, p. 367-391. In A. K. Minks and P. Harrewijn (ed.), World Crop Pests, vol. 2A. Aphids: their biology, natural enemies and control. Elsevier, Amsterdam, The Netherlands.","journal-title":"World Crop Pests, vol. 2A. Aphids: their biology, natural enemies and control"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.9.3819"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpev.2001.0983"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(01)02447-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1993.0098"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.20.6321-6324.1991"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02498635"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/35024074"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13253.x"},{"key":"e_1_3_2_16_2","first-page":"15","volume":"176","year":"2001","unstructured":"Silva, F. J., A. Latorre, and A. Moya. 2001. Genome size reduction through multiple events of gene disintegration in Buchnera APS. Trends Genet.176:15-618.","journal-title":"Trends Genet."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002849910052"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.15.4768-4777.1997"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.1.117-125.1999"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.180310197"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.68.5.2572-2575.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.68.5.2572-2575.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:49:02Z","timestamp":1645638542000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.68.5.2572-2575.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,5]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,5]]}},"alternative-id":["10.1128\/AEM.68.5.2572-2575.2002"],"URL":"https:\/\/doi.org\/10.1128\/aem.68.5.2572-2575.2002","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,5]]},"assertion":[{"value":"2001-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-02-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}