{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:24:28Z","timestamp":1793049868676,"version":"4.5.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    By the use of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    DH1 harboring\n                    <jats:italic>cphA<\/jats:italic>\n                    from\n                    <jats:italic>Synechocystis<\/jats:italic>\n                    sp. strain PCC6803, large-scale production of cyanophycin at 30- and 500-liter culture volumes was established. Transcription of\n                    <jats:italic>cphA<\/jats:italic>\n                    was controlled by the thermosensitive cI857 repressor, which enabled induction of\n                    <jats:italic>cphA<\/jats:italic>\n                    by a simple temperature shift in the culture fluid. Maximum cyanophycin cell content of up to 24% (wt\/wt) of cellular dry matter was obtained by induction in the early exponential growth phase and cultivation of the cells in terrific broth complex medium. Synthesis of cyanophycin was found to be strongly dependent on the presence of complex components, and in mineral salts medium the cells synthesized and accumulated cyanophycin only if Casamino Acids were added. Cultivations were done at the 500-liter scale, allowing the provision of cell mass for the preparation of cyanophycin at the kilogram scale. Isolation of cyanophycin was achieved by a new acid extraction procedure which allowed large-scale purification of the polyamide from whole cells.\n                  <\/jats:p>","DOI":"10.1128\/aem.68.7.3377-3384.2002","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T19:07:23Z","timestamp":1027883243000},"page":"3377-3384","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":107,"title":["Technical-Scale Production of Cyanophycin with Recombinant Strains of\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"68","author":[{"given":"Kay M.","family":"Frey","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie, Westfa\u0308lische Wilhelms-Universita\u0308t, D-48149 Mu\u0308nster"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred B.","family":"Oppermann-Sanio","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie, Westfa\u0308lische Wilhelms-Universita\u0308t, D-48149 Mu\u0308nster"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Bayer AG, D-51368 Leverkusen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Steinbu\u0308chel","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie, Westfa\u0308lische Wilhelms-Universita\u0308t, D-48149 Mu\u0308nster"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030000206"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.5.2176-2182.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bm010075a"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01622.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.12.4431"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb08849.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(97)01155-4"},{"key":"e_1_3_2_11_2","unstructured":"Hanson R. 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