{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T01:43:12Z","timestamp":1789868592371,"version":"4.0.1"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Outbreaks of listeriosis and febrile gastroenteritis have been linked to produce contamination by\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            . In order to begin to understand the physiology of the organism in a produce habitat, the ability of\n            <jats:italic>L<\/jats:italic>\n            .\n            <jats:italic>monocytogenes<\/jats:italic>\n            to attach to freshly cut radish tissue was examined. All strains tested had the capacity to attach sufficiently well such that they could not be removed during washing of the radish slices. A screen was developed to identify Tn\n            <jats:italic>917<\/jats:italic>\n            -LTV3 mutants that were defective in attachment to radish tissue, and three were characterized. Two of the three mutations were in genes with unknown functions. Both of the unknown genes mapped to a region predicted to contain genes necessary for flagellar export; however, only one of the two insertions caused a motility defect. The third insertion was found to be in an operon encoding a phosphoenolpyruvate-sugar phosphotransferase system. All three mutants were defective in attachment when tested at 30\u00b0C; the motility mutant had the most severe phenotype. However, not all of the mutants were defective when tested at other temperatures. These results indicate that\n            <jats:italic>L<\/jats:italic>\n            .\n            <jats:italic>monocytogenes<\/jats:italic>\n            may use different attachment factors at different temperatures and that temperature should be considered an important variable in studies of the molecular mechanisms of\n            <jats:italic>Listeria<\/jats:italic>\n            fitness in complex environments.\n          <\/jats:p>","DOI":"10.1128\/aem.69.1.258-266.2003","type":"journal-article","created":{"date-parts":[[2003,1,3]],"date-time":"2003-01-03T22:02:44Z","timestamp":1041631364000},"page":"258-266","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Attachment of\n            <i>Listeria monocytogenes<\/i>\n            to Radish Tissue Is Dependent upon Temperature and Flagellar Motility"],"prefix":"10.1128","volume":"69","author":[{"given":"Lisa","family":"Gorski","sequence":"first","affiliation":[{"name":"Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California 94710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D.","family":"Palumbo","sequence":"additional","affiliation":[{"name":"Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California 94710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Mandrell","sequence":"additional","affiliation":[{"name":"Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California 94710"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Albertini A. M. T. Caramori W. D. Crabb F. Scoffone and A. Galizzi. 1991. The flaA locus of Bacillus subtilis is part of a large operon coding for flagellar structures motility functions and an ATPase-like polypeptide. J. Bacteriol. 173 : 3573-3579.","DOI":"10.1128\/jb.173.11.3573-3579.1991"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1990.tb01557.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.10.3887-3894.1997"},{"key":"e_1_3_2_5_2","unstructured":"Anonymous. 20 September 2001. Boskovich fresh cut recalls fresh halved and seeded red bell peppers. SafetyAlterts.com. [Online.] http:\/\/209.58.136.120.\/recall\/f\/014\/f45110.htm\n."},{"key":"e_1_3_2_6_2","unstructured":"Anonymous. 21 September 2001. Mrs. Crockett's kitchens recalls certain potato salad products. SafetyAlerts.com. [Online.] http:\/\/209.58.136.120.\/recall\/f\/00\/crocketts.htm\n."},{"key":"e_1_3_2_7_2","unstructured":"Anonymous. 28 April 1999. 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