{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:29:41Z","timestamp":1790670581719,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Existing media designed for selective isolation of clinically important members of the genus\n            <jats:italic>Yersinia<\/jats:italic>\n            were found to be unsatisfactory for the growth and isolation of\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            . We report the development of a new selective agar medium (termed BIN) that supports the growth of\n            <jats:italic>Y. pestis<\/jats:italic>\n            . The development of the formulation of this medium was based on a fluorescence screening system designed for monitoring bacterial growth on semisolid media, using a green fluorescent protein-expressing strain. High-throughput combinatorial experiments can be conducted for the quantitative evaluation of the effect of different medium components on growth. Generation of fluorescence plots in this system, using microplates, allowed the quantitative evaluation of the growth rate of\n            <jats:italic>Y. pestis<\/jats:italic>\n            EV76 cultures in different agar compositions. The final BIN formulation is based on brain heart infusion agar, to which the selective agents irgasan, cholate salts, crystal violet, and nystatin were introduced. It was found that BIN agar is more efficient in supporting colony formation and recovery of\n            <jats:italic>Y. pestis<\/jats:italic>\n            than are the conventional semisolid media MacConkey agar and\n            <jats:italic>Yersinia<\/jats:italic>\n            -selective agar (cefsulodin-irgasan-novobiocin agar). The advantage of BIN over other media has been also demonstrated in recovering virulent\n            <jats:italic>Y. pestis<\/jats:italic>\n            from the mixed bacterial populations found in decaying carcasses of infected mice. The BIN medium is suggested as a selective medium for isolation and recovery of\n            <jats:italic>Y. pestis<\/jats:italic>\n            from various backgrounds.\n          <\/jats:p>","DOI":"10.1128\/aem.69.10.5787-5792.2003","type":"journal-article","created":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T00:30:32Z","timestamp":1065486632000},"page":"5787-5792","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Development of an Improved Selective Agar Medium for Isolation of\n            <i>Yersinia pestis<\/i>"],"prefix":"10.1128","volume":"69","author":[{"given":"Raphael","family":"Ber","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuelle","family":"Mamroud","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moshe","family":"Aftalion","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avital","family":"Tidhar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gur","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Flashner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona 74100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Aleksic S. and J. 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