{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T05:02:30Z","timestamp":1794286950898,"version":"4.9.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Kauffmann-White scheme for serotyping\n            <jats:italic>Salmonella<\/jats:italic>\n            recognizes 46 somatic (O) antigen groups, which together with detection of the flagellar (H) antigens form the basis for serotype identification. Although serotyping has become an invaluable typing method for epidemiological investigations of\n            <jats:italic>Salmonella<\/jats:italic>\n            , it does have some practical limitations. We have been characterizing the genes required for O and H antigen biosynthesis with the goal of developing a DNA-based system for the determination of serotype in\n            <jats:italic>Salmonella.<\/jats:italic>\n            The majority of the enzymes involved in O antigen biosynthesis are encoded by the\n            <jats:italic>rfb<\/jats:italic>\n            gene cluster. We report the sequencing of the\n            <jats:italic>rfb<\/jats:italic>\n            region from\n            <jats:italic>S. enterica<\/jats:italic>\n            serotype Sundsvall (serogroup O:6,14). The\n            <jats:italic>S. enterica<\/jats:italic>\n            serotype Sundsvall\n            <jats:italic>rfb<\/jats:italic>\n            region is 8.4 kb in length and comprises six open reading frames. When compared with other previously characterized\n            <jats:italic>rfb<\/jats:italic>\n            regions, the serogroup O:6,14 sequence is most related to serogroup C\n            <jats:sub>1<\/jats:sub>\n            . On the basis of DNA sequence similarity, we identified two genes from the mannose biosynthetic pathway, two mannosyl transferase genes, the O unit flippase gene and, possibly, the O antigen polymerase. The whole cluster is derived from a low-G+C-content organism. Comparative sequencing of an additional serogroup O:6,14 isolate (\n            <jats:italic>S. enterica<\/jats:italic>\n            serotype Carrau) revealed a highly homologous sequence, suggesting that O antigen factors O:24 and O:25 (additional O factors associated with serogroup O:6,14) are encoded outside the\n            <jats:italic>rfb<\/jats:italic>\n            gene cluster. We developed a serogroup O:6,14-specific PCR assay based on a region of the putative\n            <jats:italic>wzx<\/jats:italic>\n            (O antigen flippase) gene. This provides the basis for a sensitive and specific test for the rapid identification of\n            <jats:italic>Salmonella<\/jats:italic>\n            serogroup O:6,14.\n          <\/jats:p>","DOI":"10.1128\/aem.69.10.6099-6105.2003","type":"journal-article","created":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T00:30:32Z","timestamp":1065486632000},"page":"6099-6105","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Molecular Analysis of the\n            <i>rfb<\/i>\n            O Antigen Gene Cluster of\n            <i>Salmonella enterica<\/i>\n            Serogroup O:6,14 and Development of a Serogroup-Specific PCR Assay"],"prefix":"10.1128","volume":"69","author":[{"given":"Collette","family":"Fitzgerald","sequence":"first","affiliation":[{"name":"Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Sherwood","sequence":"additional","affiliation":[{"name":"Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda L.","family":"Gheesling","sequence":"additional","affiliation":[{"name":"Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances W.","family":"Brenner","sequence":"additional","affiliation":[{"name":"Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia I.","family":"Fields","sequence":"additional","affiliation":[{"name":"Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_1_3_2","unstructured":"Brenner F. W. and A. C. McWhorter-Murlin. 1998. Identification and serotyping of Salmonella. Centers for Disease Control and Prevention Atlanta Ga."},{"key":"e_1_3_1_4_2","unstructured":"Brenner F. W. 1998. Modified Kaufmann-White scheme. Centers for Disease Control and Prevention Atlanta Ga."},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb00859.x"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1574-6968.1997.tb12645.x","volume":"154","unstructured":"Collighan, R. J., and M. J. Woodward. Sequence analysis and distribution of an IS3-like insertion element isolated from Salmonella enteritidis.FEMS Microbiol. Lett.154:207-213.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.4.1002-1007.1998"},{"key":"e_1_3_1_8_2","unstructured":"Fields P. I. C. Fitzgerald and J. R. McQuiston. 2002. 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Lawrence. 1996. Phylogenetics and the amelioration of bacterial genomes p. 2627-2637. In F. C. Neidhardt R. Curtiss III J. L. Ingraham E. C. C. Lin K. B. Low B. Magasanik W. S. Reznikoff M. Riley M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella : cellular and molecular biology 2nd ed. vol. 2. ASM Press Washington D.C."},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/318832"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(01)01274-8"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-9-2291"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(97)82912-5"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5306-5314.1999"},{"key":"e_1_3_1_34_2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1017\/S0022172400034677","volume":"34","year":"1934","unstructured":"Salmonella Subcommittee. 1934. 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