{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:12:04Z","timestamp":1793059924303,"version":"4.5.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Several isolates, belonging to two new species of the same novel genus of gamma-proteobacteria, were recovered from drilled well (borehole) and spa water at Sa\u0303o Gemil in central Portugal. These organisms are phylogenetically most closely related to the strictly intracellular uncultured species of the genus\n            <jats:italic>Rickettsiella<\/jats:italic>\n            , which cause disease in arthropods, and to the facultatively intracellular species of the genus\n            <jats:italic>Legionella<\/jats:italic>\n            , some of which cause Legionnaires' disease and Pontiac fever. The Sa\u0303o Gemil strains grew only on media containing charcoal, as is also true of the species of the genus\n            <jats:italic>Legionella<\/jats:italic>\n            . Unlike the vast majority of\n            <jats:italic>Legionella<\/jats:italic>\n            isolates, the new isolates did not require\n            <jats:sc>l<\/jats:sc>\n            -cysteine or ferric pyrophosphate for growth but like the legionellae had an absolute requirement for \u03b1-ketoglutarate. Strains SGT-39\n            <jats:sup>T<\/jats:sup>\n            and SGT-56 grew consistently between 30 and 43\u00b0C, while strains SGT-108\n            <jats:sup>T<\/jats:sup>\n            and SGT-109 grew between 30 and 40\u00b0C. The pH ranges for growth of these organisms were surprisingly narrow: strains SGT-39\n            <jats:sup>T<\/jats:sup>\n            and SGT-56 grew between pH 6.3 and 7.3, while strains SGT-108\n            <jats:sup>T<\/jats:sup>\n            and SGT-109 grew between pH 6.3 and 7.0. Both organisms proliferated in the amoeba\n            <jats:italic>Hartmannella vermiformis<\/jats:italic>\n            but did not grow in U937 human cells. Based on 16S rRNA gene sequence analysis and physiological, biochemical, and chemical analysis we describe two new species of one novel genus; one species is represented by strain SGT-39\n            <jats:sup>T<\/jats:sup>\n            , for which we propose the name\n            <jats:italic>Aquicella lusitana<\/jats:italic>\n            , while strain SGT-108\n            <jats:sup>T<\/jats:sup>\n            represents a second species of the same genus, for which we propose the name\n            <jats:italic>Aquicella siphonis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.69.11.6533-6540.2003","type":"journal-article","created":{"date-parts":[[2003,11,6]],"date-time":"2003-11-06T08:42:33Z","timestamp":1068108153000},"page":"6533-6540","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Gamma-Proteobacteria\n            <i>Aquicella lusitana<\/i>\n            gen. nov., sp. nov., and\n            <i>Aquicella siphonis<\/i>\n            sp. nov. Infect Protozoa and Require Activated Charcoal for Growth in Laboratory Media"],"prefix":"10.1128","volume":"69","author":[{"given":"Paula","family":"Santos","sequence":"first","affiliation":[{"name":"Departamento de Zoologia and Centro de Neurocie\u0302ncias, Universidade de Coimbra, 3004-517 Coimbra"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Pinhal","sequence":"additional","affiliation":[{"name":"Departamento de Zoologia and Centro de Neurocie\u0302ncias, Universidade de Coimbra, 3004-517 Coimbra"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred A.","family":"Rainey","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuno","family":"Empadinhas","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica and Centro de Neurocie\u0302ncias, Universidade de Coimbra, 3001-401 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica and Centro de Neurocie\u0302ncias, Universidade de Coimbra, 3001-401 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Fields","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Benson","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anto\u0301nio","family":"Veri\u0301ssimo","sequence":"additional","affiliation":[{"name":"Departamento de Zoologia and Centro de Neurocie\u0302ncias, Universidade de Coimbra, 3004-517 Coimbra"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milton S.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica and Centro de Neurocie\u0302ncias, Universidade de Coimbra, 3001-401 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1151","year":"2001","unstructured":"Adeleke, A. 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