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In order to study the transmission and spread of<jats:italic>M. conjunctivae<\/jats:italic>across domestic and wild Caprinae populations, we developed a molecular method for subtyping and identifying strains of<jats:italic>M. conjunctivae<\/jats:italic>. This method is based on DNA sequence determination of a variable domain within the gene<jats:italic>lppS<\/jats:italic>, a gene that encodes an antigenic lipoprotein of<jats:italic>M. conjunctivae<\/jats:italic>. This domain of<jats:italic>lppS<\/jats:italic>shows variations among different strains but remains constant upon generations of individual strains on growth medium and thus allows identification of individual strains and estimation of their phylogenetic intercorrelations. The variable domain of<jats:italic>lppS<\/jats:italic>is amplified by PCR using primers that match conserved sequences of<jats:italic>lppS<\/jats:italic>flanking it. Sequence analysis of the amplified fragment enables fine subtyping of<jats:italic>M. conjunctivae<\/jats:italic>strains. The method is applicable both to isolated strains and to clinical samples directly without requiring the cultivation of the strain. Using this method, we show that<jats:italic>M. conjunctivae<\/jats:italic>was transmitted between domestic and wild animals that were grazing in proximate pastures. Certain animals also presented infections with two different strains simultaneously.<\/jats:p>","DOI":"10.1128\/aem.69.4.1913-1919.2003","type":"journal-article","created":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T06:42:22Z","timestamp":1049438542000},"page":"1913-1919","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Molecular Epidemiology of<i>Mycoplasma conjunctivae<\/i>in Caprinae: Transmission across Species in Natural Outbreaks"],"prefix":"10.1128","volume":"69","author":[{"given":"Luc","family":"Belloy","sequence":"first","affiliation":[{"name":"Institute of Veterinary Bacteriology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Janovsky","sequence":"additional","affiliation":[{"name":"Center for Fish and Wildlife Health, Institute of Animal Pathology, University of Berne, CH-3012 Berne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edy M.","family":"Vilei","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Pilo","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Giacometti","sequence":"additional","affiliation":[{"name":"Wildvet Projects, CH-7605 Stampa, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Frey","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00192-8"},{"key":"e_1_3_2_3_2","unstructured":"Ausubel F. 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