{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:54:36Z","timestamp":1791035676344,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            One limitation of employing\n            <jats:italic>lux<\/jats:italic>\n            bioreporters to monitor in situ microbial gene expression in dynamic, laboratory-scale systems is the confounding variability in the luminescent responses. For example, despite careful control of oxygen tension, growth stage, and cell number, luminescence from\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            RB1353, a naphthalene-degrading\n            <jats:italic>lux<\/jats:italic>\n            bioreporter, varied by more than sevenfold during saturated flow column experiments in our laboratory. Therefore, this study was conducted to determine what additional factors influence the luminescent response. Specifically, this study investigated the impact of temperature, pH, and initial cell number (variations within an order of magnitude) on the peak luminescence of\n            <jats:italic>P. putida<\/jats:italic>\n            RB1353 and the maximum degradation rate (\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>max<\/jats:sub>\n            ) during salicylate and naphthalene catabolism. Statistical analyses based on general linear models indicated that under constant oxygen tension, temperature and pH accounted for 98.1% of the variability in luminescence during salicylate catabolism and 94.2 and 49.5% of the variability in\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>max<\/jats:sub>\n            during salicylate and naphthalene catabolism, respectively. Temperature, pH, and initial substrate concentration accounted for 99.9% of the variability in luminescence during naphthalene catabolism. Initial cell number, within an order of magnitude, did not have a significant influence on either peak luminescence or\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>max<\/jats:sub>\n            during salicylate and naphthalene catabolism. Over the ranges of temperature and pH evaluated, peak luminescence varied by more than 4 orders of magnitude. The minimum parameter deviation required to alter\n            <jats:italic>lux<\/jats:italic>\n            gene expression during salicylate and naphthalene catabolism was a change in temperature of 1\u00b0C, a change in pH of 0.2, or a change in initial cell number of 1 order of magnitude. Results from this study indicate that there is a need for careful characterization of the impact of environmental conditions on both the expression of the reporter and catabolic genes and the activities of the gene products. For example, even though\n            <jats:italic>lux<\/jats:italic>\n            gene expression was occurring at \u223c35\u00b0C, the luciferase enzyme was inactive. Furthermore, this study demonstrates that with careful characterization and standardization of measurement conditions, the attainment of a reproducible luminescent response and an understanding of the response are feasible.\n          <\/jats:p>","DOI":"10.1128\/aem.69.4.2209-2216.2003","type":"journal-article","created":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T06:42:22Z","timestamp":1049438542000},"page":"2209-2216","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Effect of Temperature, pH, and Initial Cell Number on\n            <i>luxCDABE<\/i>\n            and\n            <i>nah<\/i>\n            Gene Expression during Naphthalene and Salicylate Catabolism in the Bioreporter Organism\n            <i>Pseudomonas putida<\/i>\n            RB1353"],"prefix":"10.1128","volume":"69","author":[{"given":"Jonathan G.","family":"Dorn","sequence":"first","affiliation":[{"name":"Department of Soil, Water and Environmental Science, University of Arizona, Tucson, Arizona 85721"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Frye","sequence":"additional","affiliation":[{"name":"Department of Soil, Water and Environmental Science, University of Arizona, Tucson, Arizona 85721"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raina M.","family":"Maier","sequence":"additional","affiliation":[{"name":"Department of Soil, Water and Environmental Science, University of Arizona, Tucson, Arizona 85721"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Agency for Toxic Substances and Disease Registry. 1995. Toxicological profile for naphthalene (update) p. 200. Agency for Toxic Substances and Disease Registry Atlanta Ga."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.6.2013-2021.1996"},{"key":"e_1_3_2_4_2","unstructured":"Burlage R. S. 1997. Emerging technologies: bioreporters biosensors and microprobes p. 115-123. In C. J. Hurst (ed.) Manual of environmental microbiology. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.9.4749-4757.1990"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr990115p"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","unstructured":"Environ. Microbiol. 2001 3","DOI":"10.1046\/j.1462-2920.2001.00190.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-3-599"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.6.1839-1846.1992"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.5.1487-1494.1994"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(98)00043-8"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/es60152a601"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac9819638"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1994.tb03058.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00384-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.42.100188.001055"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.1.123-142.1991"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3473-3482.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0160-4120(98)00037-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.11.3368-3374.1990"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/es9908319"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1515\/bchm1.1881.5.5.285"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(00)00245-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408418809104459"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.4.2209-2216.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.4.2209-2216.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:16:45Z","timestamp":1645625805000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.69.4.2209-2216.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1128\/AEM.69.4.2209-2216.2003"],"URL":"https:\/\/doi.org\/10.1128\/aem.69.4.2209-2216.2003","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]},"assertion":[{"value":"2002-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}