{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:26:53Z","timestamp":1791268013329,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            JB2 can use 2-chlorobenzoate (2-CBa), 3-CBa, 2,3-dichlorobenzoate (2,3-DCBa), and 2,5-DCBa as sole carbon and energy sources, whereas strain 142 can only grow on 2-CBa and 2,4-DCBa. Both strains, however, harbor the same halobenzoate 1,2-dioxygenase (\n            <jats:italic>ohbAB<\/jats:italic>\n            ) and chlorocatechol (\n            <jats:italic>clcABD<\/jats:italic>\n            ) degradation genes necessary for the metabolism of\n            <jats:italic>ortho<\/jats:italic>\n            -CBas. In addition, the\n            <jats:italic>hybABCD<\/jats:italic>\n            operon, encoding a salicylate 5-hydroxylase, is also found in both strains. The expression of\n            <jats:italic>ohbAB<\/jats:italic>\n            ,\n            <jats:italic>hybABCD<\/jats:italic>\n            , and\n            <jats:italic>clcABD<\/jats:italic>\n            operons was measured in cultures grown on different CBas as the sole carbon source and also in glucose-grown cells supplemented with CBas as inducers. A method to standardize real-time reverse transcription-PCR experimental data was used that allows the comparison of semiquantitative mRNA accumulation in different strains and culture conditions. In both strains, the\n            <jats:italic>ohb<\/jats:italic>\n            and\n            <jats:italic>hyb<\/jats:italic>\n            systems were induced in cells grown on 2-CBa or DCBas, whereas\n            <jats:italic>clc<\/jats:italic>\n            was induced only by DCBas. Repression by catabolite was observed both on\n            <jats:italic>ohb<\/jats:italic>\n            and\n            <jats:italic>clc<\/jats:italic>\n            systems in glucose-grown cells. Chlorocatechol 1,2-dioxygenase activity in JB2 was detected even in\n            <jats:italic>clc<\/jats:italic>\n            -repressed conditions, confirming the presence of additional isofunctional genes previously detected in\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            142. Although similar levels of induction of\n            <jats:italic>ohbAB<\/jats:italic>\n            were observed in strain JB2 grown on either benzoate, monochlorobenzoates, or DCBas, the\n            <jats:italic>ohbAB<\/jats:italic>\n            operon of strain 142 was only strongly induced by growth on 2-CBa and, to a lesser extent, on 2,4-DCBa. This observation suggests that regulation of the\n            <jats:italic>ohbAB<\/jats:italic>\n            operon may be different in both strains. The concomitant induction of\n            <jats:italic>ohb<\/jats:italic>\n            and\n            <jats:italic>hyb<\/jats:italic>\n            by CBas may allow the formation of hybrid halobenzoate dioxygenase(s) composed of Ohb\/Hyb dioxygenase subunits and Hyb ferredoxin\/ferredoxin reductase components.\n          <\/jats:p>","DOI":"10.1128\/aem.69.4.2269-2275.2003","type":"journal-article","created":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T06:42:22Z","timestamp":1049438542000},"page":"2269-2275","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Real-Time Reverse Transcription-PCR Analysis of Expression of Halobenzoate and Salicylate Catabolism-Associated Operons in Two Strains of\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"69","author":[{"given":"M. E.","family":"Corbella","sequence":"first","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Puyet","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ausubel F. M. R. Brent R. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 1991. Current protocols in molecular biology vol. 1. John Wiley & Sons Inc. 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