{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:23:54Z","timestamp":1791462234405,"version":"4.3.4"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The wine bacterium<jats:italic>Oenococcus oeni<\/jats:italic>has to cope with harsh environmental conditions, including an acidic pH, a high alcoholic content, nonoptimal growth temperatures, and growth-inhibitory compounds such as fatty acids, phenolic acids, and tannins. We describe the characterization and cloning of the<jats:italic>O. oeni ftsH<\/jats:italic>gene, encoding a protease belonging to the ATP binding cassette protein superfamily. The<jats:italic>O. oeni<\/jats:italic>FtsH protein is closest in sequence similarity to the FtsH homologue of<jats:italic>Lactococcus lactis<\/jats:italic>. The<jats:italic>O. oeni ftsH<\/jats:italic>gene proved to be stress-responsive, since its expression increased at high temperatures or under osmotic shock.<jats:italic>O. oeni<\/jats:italic>FtsH protein function was tested in an<jats:italic>Escherichia coli ftsH<\/jats:italic>mutant strain, and consistent with the<jats:italic>O. oeni ftsH<\/jats:italic>gene expression pattern, the<jats:italic>O. oeni<\/jats:italic>FtsH protein provided protection for the<jats:italic>E. coli ftsH<\/jats:italic>mutant against heat shock.<jats:italic>O. oeni<\/jats:italic>and<jats:italic>Bradyrhizobium japonicum<\/jats:italic>FtsH proteins also triggered<jats:italic>E. coli<\/jats:italic>resistance to wine toxicity. Genes homologous to<jats:italic>O. oeni ftsH<\/jats:italic>were detected in many other lactic acid bacteria found in wine, suggesting that this type of gene constitutes a well-conserved stress-protective molecular device.<\/jats:p>","DOI":"10.1128\/aem.69.5.2512-2520.2003","type":"journal-article","created":{"date-parts":[[2003,5,5]],"date-time":"2003-05-05T22:24:13Z","timestamp":1052173453000},"page":"2512-2520","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["The<i>ftsH<\/i>Gene of the Wine Bacterium<i>Oenococcus oeni<\/i>Is Involved in Protection against Environmental Stress"],"prefix":"10.1128","volume":"69","author":[{"given":"Jean-Paul","family":"Bourdineaud","sequence":"first","affiliation":[{"name":"Faculte\u0301 d'Oenologie, Universite\u0301 de Bordeaux II, 33405 Talence Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Nehme\u0301","sequence":"additional","affiliation":[{"name":"Faculte\u0301 d'Oenologie, Universite\u0301 de Bordeaux II, 33405 Talence Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Tesse","sequence":"additional","affiliation":[{"name":"Faculte\u0301 d'Oenologie, Universite\u0301 de Bordeaux II, 33405 Talence Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Lonvaud-Funel","sequence":"additional","affiliation":[{"name":"Faculte\u0301 d'Oenologie, Universite\u0301 de Bordeaux II, 33405 Talence Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37677-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950170710"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4105-4112.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.5344\/ajev.1990.41.1.48","volume":"41","year":"1990","unstructured":"Edwards, C. 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