{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:38:16Z","timestamp":1791236296086,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The three species of the group 1 bacilli,\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            ,\n            <jats:italic>B. cereus<\/jats:italic>\n            , and\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            , are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes.\n            <jats:italic>B. anthracis<\/jats:italic>\n            is the causative agent of anthrax and is phylogenetically monomorphic, while\n            <jats:italic>B. cereus<\/jats:italic>\n            and\n            <jats:italic>B. thuringiensis<\/jats:italic>\n            are genetically more diverse. An amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing\n            <jats:italic>Bacillus<\/jats:italic>\n            species, some of which show significant similarity to\n            <jats:italic>B. anthracis<\/jats:italic>\n            . Suppression subtractive hybridization was then used to characterize the genomic differences that distinguish three of the non-anthrax-causing bacilli from\n            <jats:italic>B. anthracis<\/jats:italic>\n            Ames. Ninety-three DNA sequences that were present in\n            <jats:italic>B. anthracis<\/jats:italic>\n            but absent from the non-anthrax-causing\n            <jats:italic>Bacillus<\/jats:italic>\n            genomes were isolated. Furthermore, 28 of these sequences were not found in a collection of 10 non-anthrax-causing\n            <jats:italic>Bacillus<\/jats:italic>\n            species but were present in all members of a representative collection of\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains. These sequences map to distinct loci on the\n            <jats:italic>B. anthracis<\/jats:italic>\n            genome and can be assayed simultaneously in multiplex PCR assays for rapid and highly specific DNA-based detection of\n            <jats:italic>B. anthracis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.69.5.2755-2764.2003","type":"journal-article","created":{"date-parts":[[2003,5,5]],"date-time":"2003-05-05T22:24:13Z","timestamp":1052173453000},"page":"2755-2764","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":156,"title":["Genome Differences That Distinguish\n            <i>Bacillus anthracis<\/i>\n            from\n            <i>Bacillus cereus<\/i>\n            and\n            <i>Bacillus thuringiensis<\/i>"],"prefix":"10.1128","volume":"69","author":[{"given":"Lyndsay","family":"Radnedge","sequence":"first","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter G.","family":"Agron","sequence":"additional","affiliation":[{"name":"Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen K.","family":"Hill","sequence":"additional","affiliation":[{"name":"Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence O.","family":"Ticknor","sequence":"additional","affiliation":[{"name":"Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 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