{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:19:24Z","timestamp":1790317164061,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Dehalococcoides ethenogenes<\/jats:italic>\n            is the only known cultivated organism capable of complete dehalogenation of tetrachloroethene (PCE) to ethene. The prevalence of\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            species in the environment and their association with complete dehalogenation of chloroethenes suggest that they play an important role in natural attenuation of chloroethenes and are promising candidates for engineered bioremediation of these contaminants. Both natural attenuation and bioremediation require reliable and sensitive methods to monitor the presence, distribution, and fate of the organisms of interest. Here we report the development of 16S rRNA-targeted oligonucleotide probes for\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            species. The two designed probes together encompass 28 sequences of 16S rRNA genes retrieved from the public database. Except\n            <jats:italic>D. ethenogenes<\/jats:italic>\n            and CBDB1, all the others are environmental clones obtained from sites contaminated with chlorinated ethenes. They are all closely related and form a unique cluster of\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            species. In situ hybridization of probe Dhe1259t with\n            <jats:italic>D. ethenogenes<\/jats:italic>\n            strain 195 and two enrichment cultures demonstrated the applicability of the probe to monitoring the abundance of active\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            species in these enrichment samples.\n          <\/jats:p>","DOI":"10.1128\/aem.69.5.2879-2883.2003","type":"journal-article","created":{"date-parts":[[2003,5,5]],"date-time":"2003-05-05T22:24:13Z","timestamp":1052173453000},"page":"2879-2883","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Specific Detection of\n            <i>Dehalococcoides<\/i>\n            Species by Fluorescence In Situ Hybridization with 16S rRNA-Targeted Oligonucleotide Probes"],"prefix":"10.1128","volume":"69","author":[{"given":"Yanru","family":"Yang","sequence":"first","affiliation":[{"name":"Soil Biology, Institute of Terrestrial Ecology, ETH Zurich, CH-8952 Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Zeyer","sequence":"additional","affiliation":[{"name":"Soil Biology, Institute of Terrestrial Ecology, ETH Zurich, CH-8952 Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2000.tb00557.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-3-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.11.3622-3629.1992"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.8.2287-2292.1991"},{"key":"e_1_3_2_9_2","first-page":"164","volume":"5","year":"1989","unstructured":"Felsenstein, J. 1989. PHYLIP - phylogeny inference package (version 3.2). Cladistics5:164-166.","journal-title":"Cladistics"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0016203"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.12.4973-4982.1998"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/es9805876"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.485-495.2002"},{"key":"e_1_3_2_14_2","first-page":"383","volume":"22","year":"1999","unstructured":"Holliger, C., G. Wohlfarth, and G. Diekert. 1999. Reductive dechlorination in the energy metabolism of anaerobic bacteria. FEMS Microbiol. Rev.22:383-398.","journal-title":"FEMS Microbiol. Rev."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1369-1374.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.1.109"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0157797"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00351.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/es980363n"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050486"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1998.tb00532.x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.5.2879-2883.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.5.2879-2883.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:06:24Z","timestamp":1645625184000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.69.5.2879-2883.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1128\/AEM.69.5.2879-2883.2003"],"URL":"https:\/\/doi.org\/10.1128\/aem.69.5.2879-2883.2003","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5]]},"assertion":[{"value":"2002-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}