{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T03:39:08Z","timestamp":1774669148260,"version":"3.50.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T00:00:00Z","timestamp":1051747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The goal of this study was to develop a simple plating medium to allow large-scale screening of water samples for the presence of\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            . Five conventional plating media (brain heart infusion, brucella agar, Columbia blood agar base, campylobacter agar kit Skirrow, and HPSPA medium), each containing a commercial antibiotic supplement, were initially evaluated. Eight strains selected as common waterborne organisms (\n            <jats:italic>Acinetobacter<\/jats:italic>\n            ,\n            <jats:italic>Aeromonas<\/jats:italic>\n            ,\n            <jats:italic>Bacillus<\/jats:italic>\n            ,\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ,\n            <jats:italic>Enterobacter<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus<\/jats:italic>\n            ,\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            , and\n            <jats:italic>Pseudomonas<\/jats:italic>\n            strains) were individually plated onto each of these media. Three organisms (\n            <jats:italic>Acinetobacter<\/jats:italic>\n            ,\n            <jats:italic>E. coli<\/jats:italic>\n            , and\n            <jats:italic>H. pylori<\/jats:italic>\n            ) were able to grow on all five media. This growth was unacceptable since\n            <jats:italic>Helicobacter<\/jats:italic>\n            grows very slowly and competing organisms must be inhibited for up to 7 days. Therefore, a more selective medium (HP agar) containing a novel mixture of growth supplements plus amphotericin B and polymyxin B was developed. This medium also included a phenol red color indicator for urease production. Aliquots of nonsterile well water that contained native flora (\n            <jats:italic>Flavobacterium<\/jats:italic>\n            ,\n            <jats:italic>Serratia<\/jats:italic>\n            ,\n            <jats:italic>Citrobacter<\/jats:italic>\n            ,\n            <jats:italic>Pasteurella<\/jats:italic>\n            ,\n            <jats:italic>Ochrobactrum<\/jats:italic>\n            ,\n            <jats:italic>Rahnella<\/jats:italic>\n            , and unidentified molds) and were further adulterated with the eight strains listed above (10\n            <jats:sup>6<\/jats:sup>\n            CFU of each strain per 100 ml) were spiked with\n            <jats:italic>H. pylori<\/jats:italic>\n            and were plated. In spite of the heavy mixed microbial load, only\n            <jats:italic>H. pylori<\/jats:italic>\n            colonies grew during 7 days of incubation at 37\u00b0C. The color indicator system allowed presumptive identification of\n            <jats:italic>H. pylori<\/jats:italic>\n            colonies sooner (12 to 20 h) than the conventional media tested allowed. The HP formulation developed in this study provides a medium with superior selectivity for\n            <jats:italic>H. pylori<\/jats:italic>\n            from mixed microbial populations in water and reduces the time required to complete the assay.\n          <\/jats:p>","DOI":"10.1128\/aem.69.5.2914-2918.2003","type":"journal-article","created":{"date-parts":[[2003,5,5]],"date-time":"2003-05-05T22:24:13Z","timestamp":1052173453000},"page":"2914-2918","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Development of a Plating Medium for Selection of\n            <i>Helicobacter pylori<\/i>\n            from Water Samples"],"prefix":"10.1128","volume":"69","author":[{"given":"A. J.","family":"Degnan","sequence":"first","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin\u2014Madison, Madison, Wisconsin 53718"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. C.","family":"Sonzogni","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin\u2014Madison, Madison, Wisconsin 53718"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Standridge","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin\u2014Madison, Madison, Wisconsin 53718"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199601000-00001"},{"key":"e_1_3_2_3_2","unstructured":"Baron E. J. L. R. Peterson and S. M. Finegold. 1994. Bailey & Scott's diagnostic microbiology 9th ed. Mosby-Year Book Inc. 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