{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:41:29Z","timestamp":1694623289958},"reference-count":54,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater processing is unclear. The purpose of this study was to investigate this relationship. The influence of wastewater treatment processes on enterobacterial community physiology was examined at the single-cell level by using culture-independent methods. Intracellular concentrations of two conserved proteins, the growth-related protein Fis and the stationary-phase protein Dps, were analyzed by epifluoresence microscopy of uncultivated cells by using enterobacterial group-specific polyclonal fluorochrome-coupled antibodies. Enterobacterial single-cell community protein profiles were distinct for different types of biological treatment. The differences were not apparent when bulk methods of protein analysis were used. Trickling filter wastewater yielded Fis-enriched communities compared to the communities in submerged aeration basin wastewater. Community differences in Fis and Dps contents were used to predict disinfection efficiency. Disinfection of community samples by heat exposure combined with cultivation in selective media confirmed that enterobacterial communities exhibited significant differences in sensitivity to disinfection. These findings provide strategies that can be used to increase treatment plant performance, reduce the enterobacterial content in municipal wastewater, and minimize the release of disinfection by-products into receiving water.<\/jats:p>","DOI":"10.1128\/aem.69.7.4227-4235.2003","type":"journal-article","created":{"date-parts":[[2003,7,2]],"date-time":"2003-07-02T22:00:09Z","timestamp":1057183209000},"page":"4227-4235","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Single-Cell Protein Profiling of Wastewater Enterobacterial Communities Predicts Disinfection Efficiency"],"prefix":"10.1128","volume":"69","author":[{"given":"Gomathinayagam","family":"Ponniah","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronda","family":"Michielutti","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Salonen","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Blum","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Nebraska\u2014Lincoln, Lincoln, Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.6.12b.2646"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6361-6370.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.24.8043-8056.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.44.4.814-819.1982"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.7.2993-3001.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.3.1107-1117.1997"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.7.2503-2512.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1998.0702"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.21.6145-6150.1996"},{"key":"e_1_3_2_11_2","unstructured":"Eaten A. D. L. S. Clesceri and A. E. Greenberg (ed.). 1995. Standard methods for the examination of water and wastewater 19th ed. American Public Health Association Washington D.C."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(97)80078-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.2.720-726.1988"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.3.799-802.1988"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.10.3484"},{"key":"e_1_3_2_16_2","first-page":"125","volume":"25","year":"1992","unstructured":"Kappeler, J., and W. Gujer. 1992. Estimation of kinetic parameters of heterotrophic biomass under aerobic conditions and characterization of wastewater for activated sludge modeling. Water Sci. Technol.25:125-139.","journal-title":"Water Sci. Technol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.12.4237"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.20.6433-6440.1993"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.3.649-654.1988"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.12.4658-4662.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.13.3928-3935.1994"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80121-9"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.7.2293-2298.1993"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","unstructured":"Mason C. and T. Egli. 1993. Dynamics of microbial growth in the decelerating and stationary phase of batch culture p. 81-102. In S. Kjelleber (ed.) Starvation in bacteria. Plenum Press New York N.Y.","DOI":"10.1007\/978-1-4899-2439-1_4"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb01819.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","unstructured":"Matin A. and S. Harakeh. 1990. Effect of starvation on bacterial resistance to disinfectants p. 88-103. In G. A. McFeters (ed.) Drinking water microbiology. Springer-Verlag New York N.Y.","DOI":"10.1007\/978-1-4612-4464-6_4"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(97)00061-4"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.3.921-929.1992"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.5.1465-1468.1996"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1999.0003"},{"key":"e_1_3_2_31_2","first-page":"17","volume":"43","year":"2001","unstructured":"Ogoshi, M., Y. Suzuki, and T. Asano. 2001. Water reuse in Japan. Water Sci. Technol.43:17-23.","journal-title":"Water Sci. Technol."},{"key":"e_1_3_2_32_2","unstructured":"Olsen B. and M. Stewart. 1987. Factors that change bacterial resistance to disinfection p. 885-904. In R. L. Jolley L. W. Condie J. D Johnson S. Katz R. A. Minear J. S. Mattice and V. A Jacobs (ed.) Water chlorination: chemistry environmental impact and health effects vol. 6. Lewis Publishers Inc. Chelsea Mich."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.40.100186.002005"},{"key":"e_1_3_2_34_2","first-page":"145","volume":"46","year":"2002","unstructured":"Onuki, M., H. Satoh, and T. Mino. 2002. Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate. Water Sci. Technol.46:145-154.","journal-title":"Water Sci. Technol."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1980.25.5.0943"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.5.1354-1360.1993"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1995.0576"},{"key":"e_1_3_2_38_2","unstructured":"Rittman B. E. and P. L. McCarty (ed.). 2001. Environmental biotechnology: principles and applications. McGraw-Hill Higher Education. New York N.Y."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.13.3695-3703.1995"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.4.846-854.1998"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.9.4181-4188.1999"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-1-249"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.42.5.918-920.1981"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.8.2649-2653.1992"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1994.tb00063.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90516-2"},{"key":"e_1_3_2_47_2","unstructured":"U.S. Environmental Protection Agency. 1995. National pollutant discharge elimination system permit application requirements for publicly owned treatment works and other treatment works treating domestic sewage. Fed. Regist. 60: 62562-62569."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0213-9111(01)71517-8"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2430-2435.1990"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.5.1520-1525.1993"},{"key":"e_1_3_2_51_2","first-page":"15","volume":"29","year":"1994","unstructured":"Wagner, M., R. Amaan, H. Lemmer, W. Manz, and K. H. Schleifer. 1994. Probing activated sludge with fluorescently labeled rRNA targeted oligonucleotides. Water Sci. Technol.29:15-23.","journal-title":"Water Sci. Technol."},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.3.792-800.1994"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00351.x"},{"key":"e_1_3_2_54_2","first-page":"289","volume":"46","year":"2002","unstructured":"Xu, P., F. Brissaud, J. C. Maihol, F. Valette, and V. Lazarova. 2002. Design of climate-dependent water reuse project. Water Sci. Technol.46:289-296.","journal-title":"Water Sci. Technol."},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-12-2823"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.7.4227-4235.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.7.4227-4235.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:20:07Z","timestamp":1645626007000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.69.7.4227-4235.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":54,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1128\/AEM.69.7.4227-4235.2003"],"URL":"https:\/\/doi.org\/10.1128\/aem.69.7.4227-4235.2003","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]},"assertion":[{"value":"2002-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}