{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:41:34Z","timestamp":1694623294806},"reference-count":21,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Mycobacterium chelonae<\/jats:italic>\n            and\n            <jats:italic>Mycobacterium terrae<\/jats:italic>\n            were reported to be frequently present in the environment of the\n            <jats:italic>Mycobacterium bovis<\/jats:italic>\n            BCG trial area in south India. Six isolates of\n            <jats:italic>M. chelonae<\/jats:italic>\n            and four isolates of\n            <jats:italic>M. terrae<\/jats:italic>\n            obtained from different sources in this area were analyzed by pulsed-field gel electrophoresis (PFGE) to examine large-restriction-fragment (LRF) polymorphism using the chromosomal DNA digested with\n            <jats:italic>Dra<\/jats:italic>\n            I and\n            <jats:italic>Xba<\/jats:italic>\n            I restriction enzymes. With the exception of one isolate of\n            <jats:italic>M. terrae<\/jats:italic>\n            , DNA from all other isolates could be digested with\n            <jats:italic>Dra<\/jats:italic>\n            I and\n            <jats:italic>Xba<\/jats:italic>\n            I and resulted in separable fragments. Visual comparison of the LRFs showed a unique pattern for each of the isolates tested. A computer-assisted dendrogram of the percent similarity demonstrated a high degree of genetic diversity in this group of isolates. This study demonstrates that species of nontuberculous mycobacteria, particularly\n            <jats:italic>M. chelonae<\/jats:italic>\n            and\n            <jats:italic>M. terrae<\/jats:italic>\n            , can be successfully typed by their LRF pattern using PFGE, which does not require species-specific DNA probes.\n          <\/jats:p>","DOI":"10.1128\/aem.69.7.4337-4341.2003","type":"journal-article","created":{"date-parts":[[2003,7,2]],"date-time":"2003-07-02T22:00:09Z","timestamp":1057183209000},"page":"4337-4341","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Large-Restriction-Fragment Polymorphism Analysis of\n            <i>Mycobacterium chelonae<\/i>\n            and\n            <i>Mycobacterium terrae<\/i>\n            Isolates"],"prefix":"10.1128","volume":"69","author":[{"given":"J. Daisy","family":"Vanitha","sequence":"first","affiliation":[{"name":"Bacteriology Department, Tuberculosis Research Centre (ICMR), Chennai 600031"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Venkatasubramani","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625121, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Dharmalingam","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625121, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. N.","family":"Paramasivan","sequence":"additional","affiliation":[{"name":"Bacteriology Department, Tuberculosis Research Centre (ICMR), Chennai 600031"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/144.5.1153"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1829-1836.1992"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2767-2772.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.5.1136-1142.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.19.5479-5490.2002"},{"key":"e_1_3_2_7_2","first-page":"681","volume":"5","year":"1999","unstructured":"Galil, K., L. A. Miller, M. A. Yakrus, R. J. Wallace, D. G. Mosley, B. England, G. Huitt, M. M. McNeil, and B. A. Perkins. 1999. 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