{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:17Z","timestamp":1790873117218,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Real-time PCR provides a means of detecting and quantifying DNA targets by monitoring PCR product accumulation during cycling as indicated by increased fluorescence. A number of different approaches can be used to generate the fluorescence signal. Three approaches\u2014SYBR Green I (a double-stranded DNA intercalating dye), 5\u2032-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)\u2014were evaluated for use in a real-time PCR assay to detect\n            <jats:italic>Brucella abortus<\/jats:italic>\n            . The three assays utilized the same amplification primers to produce an identical amplicon. This amplicon spans a region of the\n            <jats:italic>B. abortus<\/jats:italic>\n            genome that includes portions of the\n            <jats:italic>alkB<\/jats:italic>\n            gene and the IS\n            <jats:italic>711<\/jats:italic>\n            insertion element. All three assays were of comparable sensitivity, providing a linear assay over 7 orders of magnitude (from 7.5 ng down to 7.5 fg). However, the greatest specificity was achieved with the hybridization probe assay.\n          <\/jats:p>","DOI":"10.1128\/aem.69.8.4753-4759.2003","type":"journal-article","created":{"date-parts":[[2003,8,5]],"date-time":"2003-08-05T22:09:55Z","timestamp":1060121395000},"page":"4753-4759","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Real-Time PCR Detection of\n            <i>Brucella abortus<\/i>\n            : a Comparative Study of SYBR Green I, 5\u2032-Exonuclease, and Hybridization Probe Assays"],"prefix":"10.1128","volume":"69","author":[{"given":"D. T.","family":"Newby","sequence":"first","affiliation":[{"name":"Biotechnology Department, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho 83415"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. L.","family":"Hadfield","sequence":"additional","affiliation":[{"name":"Armed Forces Institute of Pathology, Washington, D.C. 20306"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. F.","family":"Roberto","sequence":"additional","affiliation":[{"name":"Biotechnology Department, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho 83415"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","unstructured":"Anonymous. 1997. Brucellosis. [Online.] World Health Organization Geneva Switzerland. www.who.int\/inf-fs\/en\/fact173.html"},{"key":"e_1_3_2_4_2","first-page":"271","volume":"95","year":"1992","unstructured":"Bailey, G. G., J. B. Krahn, B. S. Drasar, and N. G. Stoker. 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg.95:271-275.","journal-title":"J. Trop. Med. Hyg."},{"key":"e_1_3_2_5_2","first-page":"300","volume":"132","year":"1996","unstructured":"Barrera-Salda\u00f1a, H. A., A. M. Sifuentes-Rinc\u00f3n, and A. Revol-de Mendoza. 1996. Diagnostic\u00f3 de brucelosis por la reacci\u00f3n en cadena de la polimerasa. Gac. Med. Mex.132:300-302.","journal-title":"Gac. Med. Mex."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.6.1640-1642.1995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1996.tb04328.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221575398"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.8.3085-3086.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1990.tb01512.x"},{"key":"e_1_3_2_13_2","unstructured":"Franz D. R. P. B. Jahrling A. M. Friedlander D. J. McClain D. L. Hoover W. R. Byrne J. A. Pavlin G. W. Christopher and E. M. Eitzen Jr. 1999. Clinical recognition and management of patients exposed to biological warfare agents p. 37-79. In J. Lederberg (ed.) Biological weapons: limiting the threat. Belfer Center for Science and International Affairs Cambridge Mass."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.147.22.634"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.12.6637-6640.1990"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.6.2099-2101.1992"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3087-3090.1995"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00200-5"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3569-3576.1990"},{"key":"e_1_3_2_20_2","first-page":"69","volume":"24","year":"1980","unstructured":"Nicoletti, P. 1980. The epidemiology of bovine brucellosis. Adv. Vet. Sci. Comp. Med.24:69-98.","journal-title":"Adv. Vet. Sci. Comp. Med."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1996.tb04493.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","unstructured":"Rasmussen R. 2001. Quantification on the LightCycler p. 21-34. In S. Meuer C. Wittwer and K.-I. Nakagawara (ed.) Rapid cycle real-time PCR. Springer New York N.Y.","DOI":"10.1007\/978-3-642-59524-0_3"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2000.0338"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.5.1683-1688.1996"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3735-3737.1999"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.2144\/99276bc04"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2602-2610.2000"},{"key":"e_1_3_2_28_2","first-page":"69","volume":"88","year":"2000","unstructured":"Tcherneva, E., N. P. Rijpens, B. Jersek, and L. M. Herman. 2000. Differentiation of Brucella species by random amplified polymorphic DNA analysis. J. Appl. Microbiol.88:69-80.","journal-title":"J. Appl. Microbiol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-3-759"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-35-3-292"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0769-2609(87)90199-2"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.9.3985-3993.2001"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.2144\/97221bi01"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.8.4753-4759.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.69.8.4753-4759.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:23:36Z","timestamp":1645626216000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.69.8.4753-4759.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1128\/AEM.69.8.4753-4759.2003"],"URL":"https:\/\/doi.org\/10.1128\/aem.69.8.4753-4759.2003","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8]]},"assertion":[{"value":"2002-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-05-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}