{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T11:11:46Z","timestamp":1794049906135,"version":"4.8.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sulfate-reducing bacteria (SRB) in anoxic waters and sediments are the major producers of methylmercury in aquatic systems. Although a considerable amount of work has addressed the environmental factors that control methylmercury formation and the conditions that control bioavailability of inorganic mercury to SRB, little work has been undertaken analyzing the biochemical mechanism of methylmercury production. The acetyl-coenzyme A (CoA) pathway has been implicated as being key to mercury methylation in one SRB strain,\n            <jats:italic>Desulfovibrio desulfuricans<\/jats:italic>\n            LS, but this result has not been extended to other SRB species. To probe whether the acetyl-CoA pathway is the controlling biochemical process for methylmercury production in SRB, five incomplete-oxidizing SRB strains and two\n            <jats:italic>Desulfobacter<\/jats:italic>\n            strains that do not use the acetyl-CoA pathway for major carbon metabolism were assayed for methylmercury formation and acetyl-CoA pathway enzyme activities. Three of the SRB strains were also incubated with chloroform to inhibit the acetyl-CoA pathway. So far, all species that have been found to have acetyl-CoA activity are complete oxidizers that require the acetyl-CoA pathway for basic metabolism, as well as methylate mercury. Chloroform inhibits Hg methylation in these species either by blocking the methylating enzyme or by indirect effects on metabolism and growth. However, we have identified four incomplete-oxidizing strains that clearly do not utilize the acetyl-CoA pathway either for metabolism or mercury methylation (as confirmed by the absence of chloroform inhibition). Hg methylation is thus independent of the acetyl-CoA pathway and may not require vitamin B\n            <jats:sub>12<\/jats:sub>\n            in some and perhaps many incomplete-oxidizing SRB strains.\n          <\/jats:p>","DOI":"10.1128\/aem.69.9.5414-5422.2003","type":"journal-article","created":{"date-parts":[[2003,9,4]],"date-time":"2003-09-04T00:12:40Z","timestamp":1062634360000},"page":"5414-5422","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":134,"title":["Mercury Methylation Independent of the Acetyl-Coenzyme A Pathway in Sulfate-Reducing Bacteria"],"prefix":"10.1128","volume":"69","author":[{"given":"Eileen B.","family":"Ekstrom","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois M. 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M. C. C. Gilmour A. Heyes R. P. Mason and C. L. Miller. 2003. Geochemical and biological controls over methylmercury production and degradation in aquatic ecosystems p. 262-297. In Y. Cai and O. C. Braids (ed.) Biogeochemistry of environmentally important trace elements. 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