{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:27:29Z","timestamp":1792445249900,"version":"4.5.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We studied the potential inhibitory effect of\n            <jats:italic>Lactobacillus casei<\/jats:italic>\n            strain Shirota (from the fermented milk product Yakult [Yakult Ltd., Tokyo, Japan]) on\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            by using (i) in vitro inhibition assays with\n            <jats:italic>H. pylori<\/jats:italic>\n            SS1 (Sydney strain 1) and nine\n            <jats:italic>H. pylori<\/jats:italic>\n            clinical isolates and (ii) the in vivo\n            <jats:italic>H. pylori<\/jats:italic>\n            SS1 mouse model of infection over a period of 9 months. In vitro activity against\n            <jats:italic>H. pylori<\/jats:italic>\n            SS1 and all of the clinical isolates was observed in the presence of viable\n            <jats:italic>L. casei<\/jats:italic>\n            strain Shirota cells but not in the cell-free culture supernatant, although there was profound inhibition of urease activity. In vivo experiments were performed by oral administration of\n            <jats:italic>L. casei<\/jats:italic>\n            strain Shirota in the water supply over a period of 9 months to 6-week-old C57BL\/6 mice previously infected with\n            <jats:italic>H. pylori<\/jats:italic>\n            SS1 (study group;\n            <jats:italic>n<\/jats:italic>\n            = 25). Appropriate control groups of\n            <jats:italic>H. pylori<\/jats:italic>\n            -infected but untreated animals (\n            <jats:italic>n<\/jats:italic>\n            = 25) and uninfected animals given\n            <jats:italic>L. casei<\/jats:italic>\n            strain Shirota (\n            <jats:italic>n<\/jats:italic>\n            = 25) also were included in the study.\n            <jats:italic>H. pylori<\/jats:italic>\n            colonization and development of gastritis were assessed at 1, 2, 3, 6, and 9 months postinfection. A significant reduction in the levels of\n            <jats:italic>H. pylori<\/jats:italic>\n            colonization was observed in the antrum and body mucosa in vivo in the lactobacillus-treated study group, as assessed by viable cultures, compared to the levels in the\n            <jats:italic>H. pylori<\/jats:italic>\n            -infected control group. This reduction was accompanied by a significant decline in the associated chronic and active gastric mucosal inflammation observed at each time point throughout the observation period. A trend toward a decrease in the anti-\n            <jats:italic>H. pylori<\/jats:italic>\n            immunoglobulin G response was measured in the serum of the animals treated with lactobacillus, although this decrease was not significant.\n          <\/jats:p>","DOI":"10.1128\/aem.70.1.518-526.2004","type":"journal-article","created":{"date-parts":[[2004,1,7]],"date-time":"2004-01-07T21:02:54Z","timestamp":1073509374000},"page":"518-526","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":262,"title":["In Vitro and In Vivo Inhibition of\n            <i>Helicobacter pylori<\/i>\n            by\n            <i>Lactobacillus casei<\/i>\n            Strain Shirota"],"prefix":"10.1128","volume":"70","author":[{"given":"D.","family":"Sgouras","sequence":"first","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Maragkoudakis","sequence":"additional","affiliation":[{"name":"Laboratory of Dairy Research, Agricultural University of Athens"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Petraki","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Hippocration Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Martinez-Gonzalez","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Eriotou","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Michopoulos","sequence":"additional","affiliation":[{"name":"Gastroenterology Clinic, Alexandra Hospital, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Kalantzopoulos","sequence":"additional","affiliation":[{"name":"Laboratory of Dairy Research, Agricultural University of Athens"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Tsakalidou","sequence":"additional","affiliation":[{"name":"Laboratory of Dairy Research, Agricultural University of Athens"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0391.","family":"Mentis","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1998.00600.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.5.2001-2005.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.6.1751-1760.2001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.4.626"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2003.01452.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.1046"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4573-4580.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(01)00699-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199610000-00001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.720"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8553(05)70130-8"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.4.1349-1355.1998"},{"key":"e_1_3_2_14_2","first-page":"292","volume":"82","year":"1987","unstructured":"Hazell, S. 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