{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:08:09Z","timestamp":1762520889776},"reference-count":12,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            N304 of\n            <jats:italic>Streptomyces clavuligerus<\/jats:italic>\n            deacetoxycephalosporin C synthase was mutagenized to alter its catalytic ability. Given that N304A, N304K, N304L, and N304R mutant enzymes exhibited significant improvements in penicillin analogue conversions, we advocate that replacement of N304 with residues with aliphatic or basic side chains is preferable for engineering of a hypercatalytic enzyme.\n          <\/jats:p>","DOI":"10.1128\/aem.70.1.607-609.2004","type":"journal-article","created":{"date-parts":[[2004,1,7]],"date-time":"2004-01-07T21:02:54Z","timestamp":1073509374000},"page":"607-609","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["A Complete Library of Amino Acid Alterations at N304 in\n            <i>Streptomyces clavuligerus<\/i>\n            Deacetoxycephalosporin C Synthase Elucidates the Basis for Enhanced Penicillin Analogue Conversion"],"prefix":"10.1128","volume":"70","author":[{"given":"Hong Soon","family":"Chin","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine"},{"name":"Tropical Marine Science Institute, National University of Singapore, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kian Sim","family":"Goo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. S.","family":"Sim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00629-0"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5552"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2003.tb11525.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(00)82098-2"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4649"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2594"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.4065\/74.2.187"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/a902197c"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01957-0"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(01)00377-5"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/78467"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.4.2306-2312.2003"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.70.1.607-609.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.70.1.607-609.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:43:12Z","timestamp":1645627392000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.70.1.607-609.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1128\/AEM.70.1.607-609.2004"],"URL":"https:\/\/doi.org\/10.1128\/aem.70.1.607-609.2004","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2003-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}