{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:11:35Z","timestamp":1785363095786,"version":"3.55.0"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by\n            <jats:italic>Edwardsiella tarda<\/jats:italic>\n            , in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of\n            <jats:italic>E. tarda<\/jats:italic>\n            in 45 min at 65\u00b0C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infected Japanese flounder kidney, and spleen and seawater cultures was possible. We have developed a rapid and sensitive diagnostic protocol for edwardsiellosis detection in fish. This is the first report of the application of LAMP for the diagnosis of a fish pathogen.\n          <\/jats:p>","DOI":"10.1128\/aem.70.1.621-624.2004","type":"journal-article","created":{"date-parts":[[2004,1,7]],"date-time":"2004-01-07T21:02:54Z","timestamp":1073509374000},"page":"621-624","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":80,"title":["Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method"],"prefix":"10.1128","volume":"70","author":[{"given":"Ram","family":"Savan","sequence":"first","affiliation":[{"name":"United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arisa","family":"Igarashi","sequence":"additional","affiliation":[{"name":"United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Ehime Prefectural Chuyo Fisheries Experimental Station, Iyo City, Ehime 799-3125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Sakai","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Miyazaki University, Miyazaki 889-2192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"169","volume":"37","year":"1998","unstructured":"Chen, J. D., and S. Y. Lai. 1998. PCR for direct detection of Edwardsiella tarda from infected fish and environmental water by application of the hemolysin gene. Zool. Stud.37:169-176.","journal-title":"Zool. Stud."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050290"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3971760.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.2331\/suisan.53.395"},{"key":"e_1_3_1_6_2","unstructured":"Kusuda R. and N. Kitadai. 1993. Hemolysin production by Edwardsiella tarda isolated from eel Anguilla japonica . Jpn. Soc. Aquacult. Res. 41 : 251-255."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.5023-5028.2003"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5921"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0415"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6334"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.9.1742"},{"key":"e_1_3_1_12_2","unstructured":"Nucleic Acids Res. 2000 28"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.18.65"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1989.00390010170025"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.70.1.621-624.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.70.1.621-624.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:37:21Z","timestamp":1645627041000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.70.1.621-624.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1128\/AEM.70.1.621-624.2004"],"URL":"https:\/\/doi.org\/10.1128\/aem.70.1.621-624.2004","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2003-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-10-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}