{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:29:17Z","timestamp":1790922557680,"version":"4.1.0"},"reference-count":86,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Sodium lactate additions to a trichloroethene (TCE) residual source area in deep, fractured basalt at a U.S. Department of Energy site have resulted in the enrichment of the indigenous microbial community, the complete dechlorination of nearly all aqueous-phase TCE to ethene, and the continued depletion of the residual source since 1999. The bacterial and archaeal consortia in groundwater obtained from the residual source were assessed by using PCR-amplified 16S rRNA genes. A clone library of bacterial amplicons was predominated by those from members of the class<jats:italic>Clostridia<\/jats:italic>(57 of 93 clones), of which a phylotype most similar to that of the homoacetogen<jats:italic>Acetobacterium<\/jats:italic>sp. strain HAAP-1 was most abundant (32 of 93 clones). The remaining<jats:italic>Bacteria<\/jats:italic>consisted of phylotypes affiliated with<jats:italic>Sphingobacteria<\/jats:italic>,<jats:italic>Bacteroides<\/jats:italic>,<jats:italic>Spirochaetes<\/jats:italic>,<jats:italic>Mollicutes<\/jats:italic>, and<jats:italic>Proteobacteria<\/jats:italic>and candidate divisions OP11 and OP3. The two proteobacterial phylotypes were most similar to those of the known dechlorinators<jats:italic>Trichlorobacter thiogenes<\/jats:italic>and<jats:italic>Sulfurospirillum multivorans<\/jats:italic>. Although not represented by the bacterial clones generated with broad-specificity bacterial primers, a<jats:italic>Dehalococcoides<\/jats:italic>-like phylotype was identified with genus-specific primers. Only four distinct phylotypes were detected in the groundwater archaeal library, including predominantly a clone affiliated with the strictly acetoclastic methanogen<jats:italic>Methanosaeta concilii<\/jats:italic>(24 of 43 clones). A mixed culture that completely dechlorinates TCE to ethene was enriched from this groundwater, and both communities were characterized by terminal restriction fragment length polymorphism (T-RFLP). According to T-RFLP, the laboratory enrichment community was less diverse overall than the groundwater community, with 22 unique phylotypes as opposed to 43 and a higher percentage of<jats:italic>Clostridia<\/jats:italic>, including the<jats:italic>Acetobacterium<\/jats:italic>population. Bioreactor archaeal structure was very similar to that of the groundwater community, suggesting that methane is generated primarily via the acetoclastic pathway, using acetate generated by lactate fermentation and acetogenesis in both systems.<\/jats:p>","DOI":"10.1128\/aem.70.12.7329-7341.2004","type":"journal-article","created":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T20:18:56Z","timestamp":1101932336000},"page":"7329-7341","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":107,"title":["Molecular Characterization of a Dechlorinating Community Resulting from In Situ Biostimulation in a Trichloroethene-Contaminated Deep, Fractured Basalt Aquifer and Comparison to a Derivative Laboratory Culture"],"prefix":"10.1128","volume":"70","author":[{"given":"Tamzen W.","family":"Macbeth","sequence":"first","affiliation":[{"name":"Biotechnology Department, Idaho National Engineering and Environmental Laboratory"},{"name":"Department of Environmental Engineering, Idaho State University, Pocatello, Idaho"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Biotechnology Department, Idaho National Engineering and Environmental Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Spring","sequence":"additional","affiliation":[{"name":"Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn M.","family":"Petzke","sequence":"additional","affiliation":[{"name":"Biotechnology Department, Idaho National Engineering and Environmental Laboratory"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Kent S.","family":"Sorenson","sequence":"additional","affiliation":[{"name":"North Wind, Inc., Idaho Falls"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-003-4156-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_5_2","unstructured":"Arthur C. L. D. W. Potter K. D. Buchholz S. Motlagh and J. Pawliszyn. 1992. Solid-phase microextraction for the direct analysis of water: theory and practice. LC GC 10 : 656-661."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/es9606539"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.45.4.1286-1294.1983"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(00)89102-1"},{"key":"e_1_3_2_9_2","first-page":"313","volume":"30","year":"1999","unstructured":"Chin, K.-J., T. Lukow, S. Stubner, and R. Conrad. 1999. Structure and function of the methanogenic archaeal community in stable cellulose-degrading enrichment cultures at two different temperatures (15 and 30oC). FEMS Microbiol. Ecol.30:313-326.","journal-title":"FEMS Microbiol. Ecol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg039"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00411655"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.953-959.2003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5685"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2297-2301.2000"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.10.3869-3877.1998"},{"key":"e_1_3_2_16_2","unstructured":"Acetogenesis 1994"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.7.2943-2950.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.190-197.2001"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2555-2562.2003"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.11.2819-2824.1988"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030100275"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/412935"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/es960756r"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.11.4790-4797.2000"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1099\/00207713-52-6-1929","volume":"52","year":"2002","unstructured":"Finneran, K. T., H. M. Forbush, C. G. Van Praagh, and D. R. Lovley. 2002. Desulfitobacterium metallireducens sp. nov., an anaerobic bacterium that couples growth to the reduction of metals and humic acids as well as chlorinated compounds. Int. J. Syst. Evol. Microbiol.52:1929-1935.","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.9.2144-2151.1989"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/es025628j"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00016a017"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050308"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00156a012"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","unstructured":"Gottschalk G. 1986. Bacterial metabolism 2nd ed. Springer-Verlag New York N.Y.","DOI":"10.1007\/978-1-4612-1072-6"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1046\/j.1462-2920.2003.00525.x","volume":"6","year":"2004","unstructured":"Gu, A. Z., B. P. Hedlund, J. T. Staley, S. E. Strand, and H. D. Stensel. 2004. Analysis and comparison of the microbial community structures of two enrichment cultures capable of reductively dechlorinating TCE and cis-DCE. Environ. Microbiol.6:45-54.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.996-1003.2003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01717"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/es025528d"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.485-495.2002"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0110067"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0958-1669(95)80058-1"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050577"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1994.tb00141.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.13.4427-4434.1992"},{"key":"e_1_3_2_42_2","first-page":"383","volume":"22","year":"1999","unstructured":"Holliger, C., G. Wohlfarth, and G. Diekert. 1999. Reductive dechlorination in the energy metabolism of anaerobic bacteria. FEMS Microbiol. Rev.22:383-398.","journal-title":"FEMS Microbiol. Rev."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1992.tb05393.x"},{"key":"e_1_3_2_44_2","unstructured":"Mammalian protein metabolism 1969"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106217200"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.11.4108-4113.1996"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.20.6955"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(01)80295-4"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.9.4049-4056.1999"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.1.40-46.1999"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2002.tb00944.x"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02417-0"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5141-5147.2000"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2001.tb00773.x"},{"key":"e_1_3_2_55_2","first-page":"265","year":"2001","unstructured":"Martin, J. P., K. S. Sorenson, and L. N. Peterson. 2001. Favoring efficient in situ TCE dechlorination through amendment injection strategy, p. 265-272. In V. Magar, D. Fennell, J. L. Morse, B. Alleman, and A. Leeson (ed.), Anaerobic degradation of chlorinated solvents. Battelle Press, Columbus, Ohio.","journal-title":"Anaerobic degradation of chlorinated solvents"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.7.3108-3113.1999"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.1.264-265.1989"},{"key":"e_1_3_2_59_2","unstructured":"National Research Council. 2000. Research needs in subsurface science. National Academy Press Washington D.C."},{"key":"e_1_3_2_60_2","first-page":"420","volume":"177","year":"2002","unstructured":"Neumann, A., A. Seibert, T. Trescher, S. Reinhardt, G. Wohlfarth, and G. Diekert. 2002. Tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans: substrate specificity of the native enzyme and its corrinoid cofactor Arch. Microbiol.177:420-426.","journal-title":"Microbiol."},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6496(02)00465-8"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5313.734"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.9.3359-3367.1998"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.9.3798-3806.2000"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0157797"},{"key":"e_1_3_2_66_2","first-page":"406","volume":"4","year":"1987","unstructured":"Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol.4:406-425.","journal-title":"Mol. Biol. Evol."},{"key":"e_1_3_2_67_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00262203"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11204.x"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.761-765.1996"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0473-8"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.2.1020-1022.2001"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1021\/es011162d"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1021\/bk-2002-0837.ch008"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1080\/10889860091114293"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.469-472.2001"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2964-2974.2003"},{"key":"e_1_3_2_78_2","first-page":"973","volume":"23","year":"1994","unstructured":"Tandoi, V., T. D. DiStefano, P. A. Bowser, J. M. Gossett, and S. H. Zinder. 1994. Reductive dehalogenation of chlorinated ethenes and halogenated ethanes by a high-rate anaerobic enrichment culture. Environ. Sci. Technol.23:973-979.","journal-title":"Environ. Sci. Technol."},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb07224.x"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/BR.41.1.100-180.1977"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00248720"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.49.5.1080-1083.1985"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1021\/es960582d"},{"key":"e_1_3_2_84_2","unstructured":"The prokaryotes: an evolving electronic resource for the microbiological community release 3.0"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00695261"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1021\/es970105a"},{"key":"e_1_3_2_87_2","first-page":"387","year":"1994","unstructured":"Zinder, S. H. 1994. Syntrophic acetate oxidation and \u201creversible acetogenesis,\u201d p. 387-415. In H. L. Drake (ed.), Acetogenesis. Chapman and Hall, New York, N.Y.","journal-title":"Acetogenesis"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.70.12.7329-7341.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.70.12.7329-7341.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T23:00:59Z","timestamp":1734735659000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.70.12.7329-7341.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":86,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1128\/AEM.70.12.7329-7341.2004"],"URL":"https:\/\/doi.org\/10.1128\/aem.70.12.7329-7341.2004","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12]]},"assertion":[{"value":"2004-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-08-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}