{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:07:16Z","timestamp":1791317236555,"version":"4.3.1"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Francisella tularensis<\/jats:italic>\n            is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, antibiotic supplementation of enriched cysteine heart agar blood culture medium improved recovery of\n            <jats:italic>F. tularensis<\/jats:italic>\n            by 81.1%. For transport of tissues, immediate freezing yielded culture recovery rates as high as 94%.\n          <\/jats:p>","DOI":"10.1128\/aem.70.6.3733-3735.2004","type":"journal-article","created":{"date-parts":[[2004,6,7]],"date-time":"2004-06-07T23:03:20Z","timestamp":1086649400000},"page":"3733-3735","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Methods for Enhanced Culture Recovery of\n            <i>Francisella tularensis<\/i>"],"prefix":"10.1128","volume":"70","author":[{"given":"Jeannine M.","family":"Petersen","sequence":"first","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin E.","family":"Schriefer","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth L.","family":"Gage","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Montenieri","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon G.","family":"Carter","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miles","family":"Stanley","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"May C.","family":"Chu","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.1.600-606.2003"},{"key":"e_1_3_2_3_2","first-page":"688","volume":"51","year":"2002","unstructured":"Centers for Disease Control and Prevention. 2002. Public health dispatch: outbreak of tularemia among commercially distributed prairie dogs, 2002. Morb. Mortal. Wkly. Rep.51:688-689.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_4_2","first-page":"182","volume":"51","year":"2002","unstructured":"Centers for Disease Control and Prevention. 2002. Tularemia-United States, 1990-2000. Morb. Mortal. Wkly. Rep.51:182-184.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_5_2","first-page":"789","year":"2003","unstructured":"Chu, M. C., and R. Weyant. 2003. Francisella and Brucella, p. 789-797. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of clinical microbiology, 8th ed. American Society for Microbiology, Washington, D.C.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.21.2763"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.4.631-646.2002"},{"key":"e_1_3_2_8_2","first-page":"113","year":"1994","unstructured":"Hopla, C. E., and A. K. Hopla. 1994. Tularemia, p. 113-126. In G. W. Beran and J. H. Steele (ed.), Handbook of zoonoses, 2nd ed. CRC Press, Inc., Boca Raton, Fla.","journal-title":"Handbook of zoonoses"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.22-26.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-24.3.422"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.11.4.657"},{"key":"e_1_3_2_12_2","first-page":"419","volume":"10","year":"2003","unstructured":"Petersen, J. M., M. E. Schriefer, L. G. Carter, Y. Zhou, T. Sealy, D. Bawiec, B. Yockey, S. Urich, N. S. Zeidner, S. Avashia, J. Kool, J. Buck, C. Lindley, L. Celeda, J. A. Monteneiri, K. L. Gage, and M. C. Chu. 2003. Laboratory analysis of a tularemia outbreak in wild trapped, commercially traded prairie dogs: isolation of Francisella tularensis from seropositive animals. Emerg. Infect. Dis.10:419-425.","journal-title":"Emerg. Infect. 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