{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:07:35Z","timestamp":1790208455587,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Parvibaculum lavamentivorans<\/jats:italic>DS-1<jats:sup>T<\/jats:sup>utilized the commercial surfactant linear alkylbenzenesulfonate (LAS) (20 congeners with C<jats:sub>10<\/jats:sub>to C<jats:sub>13<\/jats:sub>side chains) as a carbon and energy source by shortening the side chain, and sulfophenylcarboxylates (SPCs) and similar compounds (e.g., \u03b1,\u03b2-unsaturated SPCs [SPC-2Hs]) were excreted with quantitative recovery of the sulfophenyl moiety. 2-(4-Sulfophenyl)decane (2-C10-LAS) was converted largely to 3-(4-sulfophenyl)butyrate (3-C4-SPC), as were 2-C12-LAS and 2-C14-LAS; the other products were 5-C6-SPC (SPC+2C) and 3-C4-SPC-2H. 2-C11-LAS was converted largely to 4-C5-SPC with the corresponding SPC+2C and SPC-2H; similarly, 3-C12-LAS yielded 4-C6-SPC with the corresponding SPC+2C and SPC-2H. This pattern of products confirmed that LAS is degraded by \u03c9-oxygenation and chain shortening through \u03b2-oxidation. At least nine major SPCs were formed from commercial LAS. The novel isolates<jats:italic>Comamonas testosteroni<\/jats:italic>SPB-2 and KF-1 utilized 3-C4-SPC;<jats:italic>Delftia acidovorans<\/jats:italic>SPH-1 utilized 4-C6-SPC enantioselectively. The substrate-dependent oxygen uptake of whole cells of strain SPB-2 indicated that there was inducible oxygenation of 3-C4-SPC and of 4-sulfophenol in whole cells of the strains of<jats:italic>C. testosteroni<\/jats:italic>during growth with 3-C4-SPC or 4-sulfophenol. The degradative pathways apparently involved 4-sulfocatechol and 4-sulfocatechol 1,2-dioxygenase. Strain SPB-2 and strain DS-1<jats:sup>T<\/jats:sup>grew together in LAS-salts medium, and only seven of the nine major SPCs were recovered. Strain SPB-2 utilized 3-C4-SPC, 3-C5-SPC, and 3-C4-SPC-2H. Strain SPH-1 grew together with strain DS-1<jats:sup>T<\/jats:sup>in LAS-salts medium, and a different set of seven major SPCs was recovered. Strain SPH-1 utilized 4-C6-SPC, 4-C5-SPC, 4-C6-SPC-2H, and 4-C5-SPC-2H. A three-member community consisting of strains DS-1<jats:sup>T<\/jats:sup>, SPB-2, and SPH-1 utilized four major SPCs. We inferred that this community mineralized the major SPCs derived from 8 of the 20 LAS congeners.<\/jats:p>","DOI":"10.1128\/aem.70.7.4053-4063.2004","type":"journal-article","created":{"date-parts":[[2004,7,7]],"date-time":"2004-07-07T19:39:25Z","timestamp":1089229165000},"page":"4053-4063","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Mineralization of Individual Congeners of Linear Alkylbenzenesulfonate by Defined Pairs of Heterotrophic Bacteria"],"prefix":"10.1128","volume":"70","author":[{"given":"David","family":"Schleheck","sequence":"first","affiliation":[{"name":"Department of Biology, The University of Konstanz, D-78457 Konstanz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Knepper","sequence":"additional","affiliation":[{"name":"Institute for Water Research and Water Technology, D-65201 Wiesbaden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Biology, The University of Konstanz, D-78457 Konstanz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alasdair M.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Biology, The University of Konstanz, D-78457 Konstanz"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02505.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1987.tb02454.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1515\/tsd-1998-350111","volume":"35","year":"1998","unstructured":"Cook, A. 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