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Potential explanations for the paucity of naturally occurring infections in swine, as compared to cattle, include a lack of animal-to-animal transmission so that the organism cannot be maintained within a herd, a high infectious dose, or herd management practices that prevent the maintenance of the organism in the gastrointestinal tract. We hypothesized that donor pigs infected with<jats:italic>E. coli<\/jats:italic>O157:H7 would transmit the organism to nai\u0308ve pigs. We also determined the infectious dose and whether housing pigs individually on grated floors would decrease the magnitude or duration of fecal shedding. Infected donor pigs shedding &lt;10<jats:sup>4<\/jats:sup>CFU of<jats:italic>E. coli<\/jats:italic>O157:H7 per g transmitted the organism to 6 of 12 nai\u0308ve pigs exposed to them. The infectious dose of<jats:italic>E. coli<\/jats:italic>O157:H7 for 3-month-old pigs was approximately 6 \u00d7 10<jats:sup>3<\/jats:sup>CFU. There was no difference in the magnitude and duration of fecal shedding by pigs housed individually on grates compared to those housed two per pen on cement floors. These results suggest that swine do not have an innate resistance to colonization by<jats:italic>E. coli<\/jats:italic>O157:H7 and that they could serve as a reservoir host under suitable conditions.<\/jats:p>","DOI":"10.1128\/aem.70.9.5331-5335.2004","type":"journal-article","created":{"date-parts":[[2004,9,2]],"date-time":"2004-09-02T20:15:34Z","timestamp":1094156134000},"page":"5331-5335","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Transmission and Infectious Dose of<i>Escherichia coli<\/i>O157:H7 in Swine"],"prefix":"10.1128","volume":"70","author":[{"given":"N. 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