{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:24:25Z","timestamp":1791149065725,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We used gene sequencing to determine whether clinical (sporadic, epidemic, and endemic) and environmental isolates of\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            serogroup (sg) 1 belong to specific lineages. A total of 178 clinical and environmental\n            <jats:italic>L. pneumophila<\/jats:italic>\n            sg 1 isolates, defined by pulsed-field gel electrophoresis and epidemiological data as sporadic, epidemic, or endemic, were analyzed for polymorphisms in five gene fragments. The fragments belonged to three housekeeping genes (coding for aconitase [\n            <jats:italic>acn<\/jats:italic>\n            ], aspartate-\u03b2-semialdehyde dehydrogenase [\n            <jats:italic>asd<\/jats:italic>\n            ], and RNA polymerase \u03b2 subunit [\n            <jats:italic>rpoB<\/jats:italic>\n            ]) and two surface protein genes (coding for the macrophage infectivity potentiator [\n            <jats:italic>mip<\/jats:italic>\n            ] and the major outer membrane protein [\n            <jats:italic>mompS<\/jats:italic>\n            ]). The phylogenetic tree inferred from sequence polymorphisms of the five genes identified two large clusters, one consisting of 133 poorly differentiated strains and containing two smaller clusters (10 and 2 strains) unrelated to each other and the other consisting of 42 strains. Clinical and environmental isolates could not be distinguished on this basis, and no link between genetic background and epidemiological type was found, suggesting that other factors are responsible for differences in pathogenicity.\n          <\/jats:p>","DOI":"10.1128\/aem.71.1.282-289.2005","type":"journal-article","created":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T12:21:15Z","timestamp":1105100475000},"page":"282-289","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Clinical and Environmental Isolates of\n            <i>Legionella pneumophila<\/i>\n            Serogroup 1 Cannot Be Distinguished by Sequence Analysis of Two Surface Protein Genes and Three Housekeeping Genes"],"prefix":"10.1128","volume":"71","author":[{"given":"Helena","family":"Aurell","sequence":"first","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Farge","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Meugnier","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manolo","family":"Gouy","sequence":"additional","affiliation":[{"name":"Laboratoire de Biom\u00e9trie et Biologie Evolutive, UMR CNRS 5558, Universit\u00e9 Claude Bernard, Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Forey","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Lina","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Vandenesch","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Etienne","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Jarraud","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence des Legionella, INSERM E-0230, Laboratoire de Bact\u00e9riologie, Facult\u00e9 de M\u00e9decine Laennec IFR 62, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3320-3322.2003"},{"key":"e_1_3_2_3_2","first-page":"314","volume":"44","year":"2002","unstructured":"Bumbaugh, A. 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