{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:51:55Z","timestamp":1781664715191,"version":"3.54.5"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was identified by 16S rRNA gene analysis as a strain of\n Alcaligenes faecalis<\/jats:italic>\n . The genes required for oxidation of hypophosphite and phosphite by this organism were identified by using transposon mutagenesis and include homologs of the\n ptxD<\/jats:italic>\n and\n htxA<\/jats:italic>\n genes of\n Pseudomonas stutzeri<\/jats:italic>\n WM88, which encode an NAD-dependent phosphite dehydrogenase (PtxD) and 2-oxoglutarate-dependent hypophosphite dioxygenase (HtxA). This organism also has the\n htxB<\/jats:italic>\n ,\n htxC<\/jats:italic>\n , and\n htxD<\/jats:italic>\n genes that comprise an ABC-type transporter, presumably for hypophosphite and phosphite transport. The role of these genes in reduced phosphorus metabolism was confirmed by analyzing the growth of mutants in which these genes were deleted. Sequencing data showed that\n htxA<\/jats:italic>\n ,\n htxB<\/jats:italic>\n ,\n htxC<\/jats:italic>\n , and\n htxD<\/jats:italic>\n are virtually identical to their homologs in\n P. stutzeri<\/jats:italic>\n at the DNA level, indicating that horizontal gene transfer occurred. However,\n A. faecalis ptxD<\/jats:italic>\n is very different from its\n P. stutzeri<\/jats:italic>\n homolog and represents a new\n ptxD<\/jats:italic>\n lineage. Therefore, this gene has ancient evolutionary roots in bacteria. These data suggest that there is strong evolutionary selection for the ability of microorganisms to oxidize hypophosphite and phosphite.\n <\/jats:p>","DOI":"10.1128\/aem.71.1.290-296.2005","type":"journal-article","created":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T12:21:15Z","timestamp":1105100475000},"page":"290-296","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Genetic Diversity and Horizontal Transfer of Genes Involved in Oxidation of Reduced Phosphorus Compounds by\n Alcaligenes faecalis<\/i>\n WM2072"],"prefix":"10.1128","volume":"71","author":[{"given":"Marlena M.","family":"Wilson","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William W.","family":"Metcalf","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","unstructured":"Current protocols in molecular biology 1992 vol. 1 and 2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.80.2.237-241.1960"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(79)63008-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg039"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011764200"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.35.5.937-944.1978"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92755-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.12.4645"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.08.110192.000435"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0442-2"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.91.2.578-582.1966"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1996.0001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.587-600.1991"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.21.5547-5558.1998"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.170.6.2575-2583.1988"},{"key":"e_1_3_2_18_2","unstructured":"Robertson J. G. 1991. IntelliKinetics version 1.01. Pennsylvania State University State College."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35017644"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(86)90421-3"},{"key":"e_1_3_2_21_2","first-page":"1357","volume":"1","year":"1996","unstructured":"Wanner, B. L. 1996. Phosphorus assimilation and control of the phosphate regulon, p. 1357-1381. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.","journal-title":"Escherichia coli and Salmonella: cellular and molecular biology"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.3.1186-1196.1990"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5876-5882.2004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204605200"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.14.4730-4739.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400664101"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.71.1.290-296.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.71.1.290-296.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T02:12:24Z","timestamp":1645582344000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.71.1.290-296.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1128\/AEM.71.1.290-296.2005"],"URL":"https:\/\/doi.org\/10.1128\/aem.71.1.290-296.2005","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]},"assertion":[{"value":"2004-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-08-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-01-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}