{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:46:14Z","timestamp":1791053174340,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein of\n            <jats:italic>Malassezia furfur<\/jats:italic>\n            in the presence of shampoo. We found that phage display of llama single-domain antibody fragments (VHHs) can be extended to very harsh conditions, such as the presence of shampoo containing nonionic and anionic surfactants. We selected several VHHs that bind to the cell wall protein Malf1 of\n            <jats:italic>M. furfur<\/jats:italic>\n            , a fungus implicated in causing dandruff. In addition to high stability in the presence of shampoo, these VHHs are also stable under other denaturing conditions, such as high urea concentrations. Many of the stable VHHs were found to contain arginine at position 44. Replacement of the native amino acid at position 44 with arginine in the most stable VHH that lacked this arginine resulted in a dramatic further increase in the stability. The combination of the unique properties of VHHs together with applied phage display and protein engineering is a powerful method for obtaining highly stable VHHs that can be used in a wide range of applications.\n          <\/jats:p>","DOI":"10.1128\/aem.71.1.442-450.2005","type":"journal-article","created":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T12:21:15Z","timestamp":1105100475000},"page":"442-450","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":110,"title":["Isolation of Llama Antibody Fragments for Prevention of Dandruff by Phage Display in Shampoo"],"prefix":"10.1128","volume":"71","author":[{"given":"Edward","family":"Dolk","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of Utrecht, Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"van der Vaart","sequence":"additional","affiliation":[{"name":"Unilever Research and Development Vlaardingen, Vlaardingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lutje Hulsik","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of Utrecht, Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gert","family":"Vriend","sequence":"additional","affiliation":[{"name":"CMBI, KUN, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"de Haard","sequence":"additional","affiliation":[{"name":"Unilever Research and Development Vlaardingen, Vlaardingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Spinelli","sequence":"additional","affiliation":[{"name":"Architecture et Fonction des Macromol\u00e9cules Biologiques, UMR 6098 CNRS and Universit\u00e9s Aix-Marseille I &amp; II, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Cambillau","sequence":"additional","affiliation":[{"name":"Architecture et Fonction des Macromol\u00e9cules Biologiques, UMR 6098 CNRS and Universit\u00e9s Aix-Marseille I &amp; II, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon","family":"Frenken","sequence":"additional","affiliation":[{"name":"Unilever Research and Development Vlaardingen, Vlaardingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"Verrips","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, University of Utrecht, Utrecht"},{"name":"Unilever Research and Development Vlaardingen, Vlaardingen"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0303-6987.2004.0135.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0147-9571(86)90008-1","volume":"9","year":"1986","unstructured":"Bokhout, B. 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