{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:06:28Z","timestamp":1791018388677,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacteria\nbelonging to the genus\n            <jats:italic>Wolbachia<\/jats:italic>\n            are obligatorymicroendocytobionts that infect a variety of arthropods and a majority\nof filarial nematode species, where they induce reproductive\nalterations or establish a mutualistic symbiosis. Although two whole\ngenome sequences of\n            <jats:italic>Wolbachia pipientis<\/jats:italic>\n            , for strain wMel from\n            <jats:italic>Drosophila melanogaster<\/jats:italic>\n            and strain wBm from\n            <jats:italic>Brugia\nmalayi<\/jats:italic>\n            , have been fully completed and six other genome sequencing\nprojects are ongoing\n(\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"url\" xlink:href=\"http:\/\/www.genomesonline.org\/index.cgi?want=Prokaryotic+Ongoin\">http:\/\/www.genomesonline.org\/index.cgi?want=Prokaryotic+Ongoin<\/jats:ext-link>\n            ),\ngenetic analyses of these bacteria are still scarce, mainly due to the\ninability to cultivate them outside of eukaryotic cells. Usually, a\nlarge amount of host tissue (a thousand individuals, or about\n10 g) is required in order to purify\n            <jats:italic>Wolbachia<\/jats:italic>\n            and\nextract its DNA, which is often recovered in small amounts and\ncontaminated by host cell DNA, thus hindering genomic studies. In this\nreport, we describe an efficient and reliable procedure to\nrepresentatively amplify the\n            <jats:italic>Wolbachia<\/jats:italic>\n            genome by\nmultiple-displacement amplification from limited infected host tissue\n(0.2 g or 2 \u00d7 10\n            <jats:sup>7<\/jats:sup>\n            cells). We obtained sufficient\namounts (8 to 10 \u03bcg) of DNA of suitable quality for genomic\nstudies, and we demonstrated that the amplified DNA contained all of\nthe\n            <jats:italic>Wolbachia<\/jats:italic>\n            loci targeted. In addition, our data indicated\nthat the genome of strain wRi, an obligatory endosymbiont of\n            <jats:italic>Drosophila simulans<\/jats:italic>\n            , shares a similar overall architecture\nwith its relative strain\nwMel.\n          <\/jats:p>","DOI":"10.1128\/aem.71.11.6910-6917.2005","type":"journal-article","created":{"date-parts":[[2005,11,3]],"date-time":"2005-11-03T22:08:08Z","timestamp":1131055688000},"page":"6910-6917","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Efficient Procedure for Purification of ObligateIntracellular\n            <i>Wolbachia pipientis<\/i>\n            and RepresentativeAmplification of Its Genome by Multiple-DisplacementAmplification"],"prefix":"10.1128","volume":"71","author":[{"given":"Patrick","family":"Mavingui","sequence":"first","affiliation":[{"name":"E\u0301cologie\rMicrobienne UMR CNRS 5557-USC INRA 1193"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van","family":"Tran Van","sequence":"additional","affiliation":[{"name":"E\u0301cologie\rMicrobienne UMR CNRS 5557-USC INRA 1193"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Labeyrie","sequence":"additional","affiliation":[{"name":"E\u0301cologie\rMicrobienne UMR CNRS 5557-USC INRA 1193"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwige","family":"Rance\u0300s","sequence":"additional","affiliation":[{"name":"E\u0301cologie\rMicrobienne UMR CNRS 5557-USC INRA 1193"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Vavre","sequence":"additional","affiliation":[{"name":"Biome\u0301trie et BiologieE\u0301\rvolutive UMR CNRS 5558, Universite\u0301\rClaude Bernard Lyon 1, Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Simonet","sequence":"additional","affiliation":[{"name":"E\u0301cologie\rMicrobienne UMR CNRS 5557-USC INRA 1193"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(01)00432-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)81883-X"},{"key":"e_1_3_2_4_2","first-page":"1081","volume":"1401","year":"1998","unstructured":"Bouchon, D., T. 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