{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:46:21Z","timestamp":1791474381792,"version":"4.3.4"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Dynamic changes in the transcriptional expression of the\n            <jats:italic>tceA<\/jats:italic>\n            gene, which encodes a trichloroethene reductive dehalogenase, were characterized in a\n            <jats:italic>Dehalococcoides<\/jats:italic>\n            -containing microbial enrichment culture. Expression was quantified by real-time PCR as the number of\n            <jats:italic>tceA<\/jats:italic>\n            transcripts per\n            <jats:italic>tceA<\/jats:italic>\n            gene. Expression of\n            <jats:italic>tceA<\/jats:italic>\n            increased 40-fold after chlorinated ethene-starved cells were exposed to trichloroethene (TCE),\n            <jats:italic>cis<\/jats:italic>\n            -dichloroethene (DCE), or 1,1-DCE but did not increase after exposure to tetrachloroethene or vinyl chloride. Surprisingly,\n            <jats:italic>tceA<\/jats:italic>\n            expression also increased 30-fold after cellular exposure to the nonmetabolic substrate\n            <jats:italic>trans<\/jats:italic>\n            -DCE, indicating that expression of\n            <jats:italic>tceA<\/jats:italic>\n            is induced by both growth-supporting and non-growth-supporting chlorinated ethenes. Additional experiments revealed that the level of\n            <jats:italic>tceA<\/jats:italic>\n            expression was independent of the concentration of chlorinated ethenes (sum concentrations of TCE and DCEs of 2.2 to 333 \u03bcM), the concentration of the electron donor hydrogen (concentrations of 12 nM to 17 \u03bcM), and the presence of alternate bacterial electron acceptors (5 mM concentrations of fumarate, sulfate, sulfite, thiosulfate, nitrate, or nitrite) but was highly dependent on incubation temperature.\n          <\/jats:p>","DOI":"10.1128\/aem.71.11.7145-7151.2005","type":"journal-article","created":{"date-parts":[[2005,11,3]],"date-time":"2005-11-03T22:08:08Z","timestamp":1131055688000},"page":"7145-7151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Transcriptional Expression of the\n            <i>tceA<\/i>\n            Gene in a\n            <i>Dehalococcoides-<\/i>\n            Containing Microbial Enrichment"],"prefix":"10.1128","volume":"71","author":[{"given":"David R.","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of California, Berkeley, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick K. 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