{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T23:57:08Z","timestamp":1792886228319,"version":"4.5.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The surface protein internalin A (InlA) contributes to the invasion of human intestinal epithelial cells by\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    . Screening of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    strains isolated from human clinical cases (\n                    <jats:italic>n<\/jats:italic>\n                    = 46), foods (\n                    <jats:italic>n<\/jats:italic>\n                    = 118), and healthy animals (\n                    <jats:italic>n<\/jats:italic>\n                    = 58) in the United States revealed mutations in\n                    <jats:italic>inlA<\/jats:italic>\n                    leading to premature stop codons (PMSCs) in\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    ribotypes DUP-1052A and DUP-16635A (PMSC mutation type 1), DUP-1025A and DUP-1031A (PMSC mutation type 2), and DUP-1046B and DUP-1062A (PMSC mutation type 3). While all DUP-1046B, DUP-1062A, DUP-16635A, and DUP-1031A isolates (\n                    <jats:italic>n<\/jats:italic>\n                    = 76) contained\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSCs, ribotypes DUP-1052A and DUP-1025A (\n                    <jats:italic>n<\/jats:italic>\n                    = 72) contained isolates with and without\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSCs. Western immunoblotting showed that all three\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSCs result in the production of truncated and secreted InlA. Searches of the Pathogen Tracker database, which contains subtype and source information for more than 5,000\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    isolates, revealed that the six ribotypes shown to contain isolates with\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSCs were overall more commonly isolated from foods than from human listeriosis cases.\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    strains carrying\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSCs also showed significantly (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0004) reduced invasion of Caco-2 cells compared to isolates with homologous 3\u2032\n                    <jats:italic>inlA<\/jats:italic>\n                    sequences without PMSCs. Invasion assays with an isogenic PMSC mutant further supported the observation that\n                    <jats:italic>inlA<\/jats:italic>\n                    PMSCs lead to reduced invasion of Caco-2 cells. Our data show that specific\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    subtypes which are common among U.S. food isolates but rare among human listeriosis isolates carry\n                    <jats:italic>inlA<\/jats:italic>\n                    mutations that are associated with, and possibly at least partially responsible for, an attenuated invasion phenotype.\n                  <\/jats:p>","DOI":"10.1128\/aem.71.12.8764-8772.2005","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T06:07:48Z","timestamp":1133849268000},"page":"8764-8772","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":177,"title":["Select\n                    <i>Listeria monocytogenes<\/i>\n                    Subtypes Commonly Found in Foods Carry Distinct Nonsense Mutations in\n                    <i>inlA<\/i>\n                    , Leading to Expression of Truncated and Secreted Internalin A, and Are Associated with a Reduced Invasion Phenotype for Human Intestinal Epithelial Cells"],"prefix":"10.1128","volume":"71","author":[{"given":"K. 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E.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Food Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Yeung","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, Ithaca, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wiedmann","sequence":"additional","affiliation":[{"name":"Department of Food Science"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.1.489-497.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","volume":"139","year":"1987","unstructured":"Bishop, D. K., and D. J. Hinrichs. 1987. Adoptive transfer of immunity to Listeria monocytogenes: the influence of in vitro stimulation on lymphocyte subset requirements. J. 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