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We report here the implementation of both approaches with full regulatory range in gram-positive bacteria as exemplified in\n            <jats:italic>Bacillus subtilis<\/jats:italic>\n            . A chromosomally located gene is controlled by one or two\n            <jats:italic>tet<\/jats:italic>\n            operators. The precise adjustment of regulatory windows is accomplished by adjusting\n            <jats:italic>tetR<\/jats:italic>\n            or\n            <jats:italic>revtetR<\/jats:italic>\n            expression via different promoters. The most efficient induction was 300-fold in the presence of 0.4 \u03bcM anhydrotetracycline obtained with a\n            <jats:italic>Pr-xylA-tetR<\/jats:italic>\n            fusion. Reversible 500-fold gene knockouts were obtained in\n            <jats:italic>B. subtilis<\/jats:italic>\n            after adjusting expression of revTetR by synthetically designed promoters. We anticipate that these tools will also be useful in many other gram-positive bacteria.\n          <\/jats:p>","DOI":"10.1128\/aem.71.2.728-733.2005","type":"journal-article","created":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T18:24:28Z","timestamp":1107455068000},"page":"728-733","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Tetracycline-Dependent Conditional Gene Knockout in\n            <i>Bacillus subtilis<\/i>"],"prefix":"10.1128","volume":"71","author":[{"given":"Annette","family":"Kamionka","sequence":"first","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Institut fu\u0308r Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universita\u0308t Erlangen-Nu\u0308rnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Bertram","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Institut fu\u0308r Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universita\u0308t Erlangen-Nu\u0308rnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Hillen","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Institut fu\u0308r Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universita\u0308t Erlangen-Nu\u0308rnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90495-W"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7851-7857.2001"},{"key":"e_1_3_2_4_2","first-page":"325","volume":"234","year":"2004","unstructured":"Fukiya, S., H. 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