{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T15:25:30Z","timestamp":1783437930428,"version":"3.54.6"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The phylogenetic relatedness among 12 agriculturally important species in the order\n            <jats:italic>Rhizobiales<\/jats:italic>\n            was estimated by comparative 16S rRNA and\n            <jats:italic>dnaK<\/jats:italic>\n            sequence analyses. Two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. One group consisted of\n            <jats:italic>Mesorhizobium loti<\/jats:italic>\n            and\n            <jats:italic>Mesorhizobium ciceri<\/jats:italic>\n            , and the other group consisted of\n            <jats:italic>Agrobacterium rhizogenes<\/jats:italic>\n            ,\n            <jats:italic>Rhizobium tropici<\/jats:italic>\n            ,\n            <jats:italic>Rhizobium etli<\/jats:italic>\n            , and\n            <jats:italic>Rhizobium leguminosarum<\/jats:italic>\n            . Although bootstrap support for the placement of the remaining six species varied,\n            <jats:italic>A. tumefaciens<\/jats:italic>\n            ,\n            <jats:italic>Agrobacterium rubi<\/jats:italic>\n            , and\n            <jats:italic>Agrobacterium vitis<\/jats:italic>\n            were consistently associated in the same subcluster. The three other species included\n            <jats:italic>Rhizobium galegae<\/jats:italic>\n            ,\n            <jats:italic>Sinorhizobium meliloti<\/jats:italic>\n            , and\n            <jats:italic>Brucella ovis<\/jats:italic>\n            . Among these, the placement of\n            <jats:italic>R. galegae<\/jats:italic>\n            was the least consistent, in that it was placed flanking the\n            <jats:italic>A. rhizogenes<\/jats:italic>\n            -\n            <jats:italic>Rhizobium<\/jats:italic>\n            cluster in the\n            <jats:italic>dnaK<\/jats:italic>\n            nucleotide sequence trees, while it was placed with the other three\n            <jats:italic>Agrobacterium<\/jats:italic>\n            species in the 16S rRNA and the DnaK amino acid trees. In an effort to explain the inconsistent placement of\n            <jats:italic>R. galegae<\/jats:italic>\n            , we examined polymorphic site distribution patterns among the various species. Localized runs of nucleotide sequence similarity were evident between\n            <jats:italic>R. galegae<\/jats:italic>\n            and certain other species, suggesting that the\n            <jats:italic>R. galegae<\/jats:italic>\n            genes are chimeric. These results provide a tenable explanation for the weak statistical support often associated with the phylogenetic placement of\n            <jats:italic>R. galegae<\/jats:italic>\n            , and they also illustrate a potential pitfall in the use of partial sequences for species identification.\n          <\/jats:p>","DOI":"10.1128\/aem.71.3.1328-1335.2005","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T19:19:12Z","timestamp":1109877552000},"page":"1328-1335","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Rhizobial 16S rRNA and\n            <i>dnaK<\/i>\n            Genes: Mosaicism and the Uncertain Phylogenetic Placement of\n            <i>Rhizobium galegae<\/i>"],"prefix":"10.1128","volume":"71","author":[{"given":"B. 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