{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:19:24Z","timestamp":1788556764081,"version":"build-2803163510"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Dehalococcoides ethenogenes<\/jats:italic>\n            strain 195 reductively dechlorinates tetrachloroethene (PCE) and trichloroethene (TCE) to vinyl chloride and ethene using H\n            <jats:sub>2<\/jats:sub>\n            as an electron donor. PCE- and TCE-reductive dehalogenase (RD) activities were mainly membrane associated, whereas only about 20% of the hydrogenase activity was membrane associated. Experiments with methyl viologen (MV) were consistent with a periplasmic location for the RDs or a component feeding electrons to them. The protonophore uncoupler tetrachlorosalicylanilide did not inhibit reductive dechlorination in cells incubated with H\n            <jats:sub>2<\/jats:sub>\n            and PCE and partially restored activity in cells incubated with the ATPase inhibitor\n            <jats:italic>N<\/jats:italic>\n            ,\n            <jats:italic>N<\/jats:italic>\n            \u2032-dicyclohexylcarbodiimide. Benzyl viologen or diquat (\n            <jats:italic>E<\/jats:italic>\n            <jats:sup>o<\/jats:sup>\n            \u2032 \u2248 \u2212360 mV) supported reductive dechlorination of PCE or TCE at rates comparable to MV (\u2212450 mV) in cell extracts.\n          <\/jats:p>","DOI":"10.1128\/aem.71.3.1664-1667.2005","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T19:19:12Z","timestamp":1109877552000},"page":"1664-1667","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Characterization of Hydrogenase and Reductive Dehalogenase Activities of\n            <i>Dehalococcoides ethenogenes<\/i>\n            Strain 195"],"prefix":"10.1128","volume":"71","author":[{"given":"Ivonne","family":"Nijenhuis","sequence":"first","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Zinder","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.10.2999-3005.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.8.2287-2292.1991"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0016203"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(01)95447-9"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2999-3001.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1640199"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.1.40-46.1999"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5141-5147.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.4.1270-1275.1998"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.7.3108-3113.1999"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/es001285i"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.11.3928-3933.1995"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050399"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0409-3"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00393380"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.16.4140-4145.1998"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.8.2328-2333.1996"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.29.20287"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1139\/w02-057"},{"key":"e_1_3_2_23_2","unstructured":"White D. 1995. 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