{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:21:38Z","timestamp":1792725698525,"version":"4.5.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Noroviruses (NoV), which are members of the family\n            <jats:italic>Caliciviridae<\/jats:italic>\n            , are the most important cause of outbreaks of acute gastroenteritis worldwide and are commonly found in shellfish grown in polluted waters. In the present study, we developed broadly reactive one-step TaqMan reverse transcription (RT)-PCR assays for the detection of genogroup I (GI) and GII NoV in fecal samples, as well as shellfish samples. The specificity and sensitivity of all steps of the assays were systematically evaluated, and in the final format, the monoplex assays were validated by using RNA extracted from a panel of 84 stool specimens, which included NoV strains representing 19 different genotypes (7 GI, 11 GII, and 1 GIV strains). The assays were further validated with 38 shellfish cDNA extracts previously tested by nested PCR. Comparison with a recently described real-time assay showed that our assay had significantly higher sensitivity and was at least as sensitive as the nested PCR. For stool specimens, a one-step duplex TaqMan RT-PCR assay performed as well as individual genogroup-specific monoplex assays. All other enteric viruses examined were negative, and no cross-reaction between genogroups was observed. These TaqMan RT-PCR assays provide rapid (less than 90 min), sensitive, and reliable detection of NoV and should prove to be useful for routine monitoring of both clinical and shellfish samples.\n          <\/jats:p>","DOI":"10.1128\/aem.71.4.1870-1875.2005","type":"journal-article","created":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T13:23:15Z","timestamp":1112793795000},"page":"1870-1875","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":314,"title":["Rapid and Sensitive Detection of Noroviruses by Using TaqMan-Based One-Step Reverse Transcription-PCR Assays and Application to Naturally Contaminated Shellfish Samples"],"prefix":"10.1128","volume":"71","author":[{"given":"Narayanan","family":"Jothikumar","sequence":"first","affiliation":[{"name":"Environmental Science and Engineering, University of North Carolina, Chapel Hill, North Carolina"},{"name":"Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Lowther","sequence":"additional","affiliation":[{"name":"The Centre for Environment Fisheries and Aquaculture Science, Weymouth, England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Henshilwood","sequence":"additional","affiliation":[{"name":"The Centre for Environment Fisheries and Aquaculture Science, Weymouth, England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David N.","family":"Lees","sequence":"additional","affiliation":[{"name":"The Centre for Environment Fisheries and Aquaculture Science, Weymouth, England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent R.","family":"Hill","sequence":"additional","affiliation":[{"name":"Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Vinje\u0301","sequence":"additional","affiliation":[{"name":"Environmental Science and Engineering, University of North Carolina, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.1.15-37.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2003.09.005","volume":"115","year":"2004","unstructured":"Beuret, C. 2004. Simultaneous detection of enteric viruses by multiplex real-time RT-PCR J. Virol. Methods115:1-8.","journal-title":"Virol. Methods"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.11.008"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/341085"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.3.858-863.1998"},{"key":"e_1_3_2_8_2","first-page":"841","year":"2001","unstructured":"Green, K. Y., A. Z. Kapikian, and R. M. Chanock. 2001. Human caliciviruses, p. 841-874. In D. M. Knipe, P. M. Howley, D. E. Griffin, et al. (ed.), Fields virology, 4th ed. Lippincott-Raven, Philadelphia, Pa.","journal-title":"Fields virology,"},{"key":"e_1_3_2_9_2","first-page":"51","volume":"38","year":"1998","unstructured":"Henshilwood, K., J. Green, and D. N. Lees. 1998. Monitoring the marine environment for small round structured viruses (SRSVs): a new approach to combating the transmission of these viruses by molluscan shellfish. Water Sci. Technol.38:51-56.","journal-title":"Water Sci. Technol."},{"key":"e_1_3_2_10_2","first-page":"451","year":"2003","unstructured":"Henshilwood, K., W. J. Dore, S. Anderson, and D. N. Lees. 2003. Investigation of Norwalk like virus elimination during depuration using a real time quantitative PCR, p. 451-465. In A. Villalba, J. L. Romalde, B. Reura, and R. Beiras (ed.), Molluscan shellfish safety. Conselleria de Pesca e Asuntos Maritimos da Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Galicia, Spain.","journal-title":"Molluscan shellfish safety."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10573"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(99)00114-7"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1548-1557.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.2988-2995.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1568"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00404-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(03)00169-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(02)00096-7"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.11.6541-6549.2003"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00248-8"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.8.2999-3005.1994"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.12.4418-4424.1995"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(03)00058-8"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.08.023"},{"key":"e_1_3_2_25_2","first-page":"115","year":"2001","unstructured":"Moe, C. L. 2001. Effects assessment: determinants of risk and outcome of waterborne infections, p. 115-135. In G. F. Craun, F. S. Hauchman, and D. E. Robinson (ed.), Microbial pathogens and disinfection by-products in drinking water: health effects and management of risks. ILSI Press, Washington, D.C.","journal-title":"Microbial pathogens and disinfection by-products in drinking water: health effects and management of risks."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.5.2678-2684.2004"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.10.5782-5786.2003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199712)53:4<372::AID-JMV10>3.0.CO;2-H"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4679-4685.2004"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.321"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.2144\/99276bc04"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.12.7130-7136.2003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/315578"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.11.001"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.3.610"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2595-2601.2000"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1423-1433.2003"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.71.4.1870-1875.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.71.4.1870-1875.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T15:14:41Z","timestamp":1645629281000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.71.4.1870-1875.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1128\/AEM.71.4.1870-1875.2005"],"URL":"https:\/\/doi.org\/10.1128\/aem.71.4.1870-1875.2005","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4]]},"assertion":[{"value":"2004-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}