{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:02:18Z","timestamp":1791352938876,"version":"4.3.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Carbon stable isotope fractionation of tetrachloroethene (PCE) during reductive dechlorination by whole cells and crude extracts of\n                    <jats:italic>Sulfurospirillum multivorans<\/jats:italic>\n                    and\n                    <jats:italic>Desulfitobacterium<\/jats:italic>\n                    sp. strain PCE-S and the abiotic reaction with cyanocobalamin (vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    ) was studied. Fractionation was largest during the reaction with cyanocobalamin with \u03b1C = 1.0132. Stable isotope fractionation was lower but still in a similar order of magnitude for\n                    <jats:italic>Desulfitobacterium<\/jats:italic>\n                    sp. PCE-S (\u03b1C = 1.0052 to 1.0098). The isotope fractionation of PCE during dehalogenation by\n                    <jats:italic>S. multivorans<\/jats:italic>\n                    was lower by 1 order of magnitude (\u03b1C = 1.00042 to 1.0017). Additionally, an increase in isotope fractionation was observed with a decrease in cell integrity for both strains. For\n                    <jats:italic>Desulfitobacterium<\/jats:italic>\n                    sp. strain PCE-S, the carbon stable isotope fractionation factors were 1.0052 and 1.0089 for growing cells and crude extracts, respectively. For\n                    <jats:italic>S. multivorans<\/jats:italic>\n                    , \u03b1C values were 1.00042, 1.00097, and 1.0017 for growing cells, crude extracts, and the purified PCE reductive dehalogenase, respectively. For the field application of stable isotope fractionation, care is needed as fractionation may vary by more than an order of magnitude depending on the bacteria present, responsible for degradation.\n                  <\/jats:p>","DOI":"10.1128\/aem.71.7.3413-3419.2005","type":"journal-article","created":{"date-parts":[[2005,7,7]],"date-time":"2005-07-07T02:37:11Z","timestamp":1120703831000},"page":"3413-3419","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":130,"title":["Stable Isotope Fractionation of Tetrachloroethene during Reductive Dechlorination by\n                    <i>Sulfurospirillum multivorans<\/i>\n                    and\n                    <i>Desulfitobacterium<\/i>\n                    sp. Strain PCE-S and Abiotic Reactions with Cyanocobalamin"],"prefix":"10.1128","volume":"71","author":[{"given":"Ivonne","family":"Nijenhuis","sequence":"first","affiliation":[{"name":"Departments of Isotope Biogeochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Andert","sequence":"additional","affiliation":[{"name":"Departments of Isotope Biogeochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Beck","sequence":"additional","affiliation":[{"name":"Departments of Isotope Biogeochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Ka\u0308stner","sequence":"additional","affiliation":[{"name":"Bioremediation, UFZ-Centre for Environmental Research Leipzig-Halle GmbH, Permoserstrasse 15, D-04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Diekert","sequence":"additional","affiliation":[{"name":"Institute for Microbiology, Friedrich-Schiller-Universita\u0308t, Philosophenweg 12, D-07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Hermann","family":"Richnow","sequence":"additional","affiliation":[{"name":"Departments of Isotope Biogeochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Anonymous. 1995. 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