{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:01:56Z","timestamp":1791158516713,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In silico analysis of the\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            genome revealed\n            <jats:italic>lmo0292<\/jats:italic>\n            , a gene predicted to encode a HtrA-like serine protease. A stable insertion mutant was constructed, revealing a requirement for\n            <jats:italic>htrA<\/jats:italic>\n            in the listerial response to heat, acid, and penicillin stress. Transcriptional analysis revealed that\n            <jats:italic>htrA<\/jats:italic>\n            is not induced in response to heat shock but is induced in response to low pH and penicillin G stress. Furthermore,\n            <jats:italic>htrA<\/jats:italic>\n            expression was shown to be dependent upon the LisRK two-component sensor-kinase, a system known to respond to changes in integrity of the cell envelope. In addition, we demonstrated that a second in-frame start codon, upstream of that previously annotated for\n            <jats:italic>L. monocytogenes htrA<\/jats:italic>\n            , incorporating a putative signal sequence appears to influence virulence potential. Finally, a significant virulence defect was observed for the\n            <jats:italic>htrA<\/jats:italic>\n            mutant, indicating that this gene is required for full virulence in mice. Our findings suggest that\n            <jats:italic>L. monocytogenes lmo0292<\/jats:italic>\n            encodes an HtrA-like serine protease that is not part of the classical heat shock response but is involved in stress responses and virulence.\n          <\/jats:p>","DOI":"10.1128\/aem.71.8.4241-4247.2005","type":"journal-article","created":{"date-parts":[[2005,8,5]],"date-time":"2005-08-05T21:53:12Z","timestamp":1123278792000},"page":"4241-4247","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Role for HtrA in Stress Induction and Virulence Potential in\n            <i>Listeria monocytogenes<\/i>"],"prefix":"10.1128","volume":"71","author":[{"given":"Helena M.","family":"Stack","sequence":"first","affiliation":[{"name":"Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy D.","family":"Sleator","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Bowers","sequence":"additional","affiliation":[{"name":"New York University School of Medicine, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cormac G. 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