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To improve the conversion efficiency in high concentrations of substrate and 2-propanol, selection of a PAR mutant library and the subsequent rearrangement of mutations were attempted. With only a single selection round and following the manual combination of advantageous mutations, PAR was successfully adapted for the conversion of high concentrations of substrate with concentrated 2-propanol. This method will be widely applicable for the engineering of enzymes potentially valuable for industry.<\/jats:p>","DOI":"10.1128\/aem.71.8.4713-4720.2005","type":"journal-article","created":{"date-parts":[[2005,8,5]],"date-time":"2005-08-05T21:53:12Z","timestamp":1123278792000},"page":"4713-4720","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Engineering of Phenylacetaldehyde Reductase for Efficient Substrate Conversion in Concentrated 2-Propanol"],"prefix":"10.1128","volume":"71","author":[{"given":"Yoshihide","family":"Makino","sequence":"first","affiliation":[{"name":"Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama 939-0398, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousuke","family":"Inoue","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama 939-0398, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Dairi","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama 939-0398, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuya","family":"Itoh","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama 939-0398, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1007\/s00253-003-1310-y","volume":"62","year":"2003","unstructured":"Abokitse, K., and W. 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